Derle Eda, Demir Buket Dönmez, Bahçekapılı Melike Sever, Aktaş Canan Çakır, Gürlek Onur Çağın, Türken Beyza, Kurşun Hülya Karataş
Department of Neurology, Başkent University Faculty of Medicine, Ankara, Türkiye.
Institute of Neurological Sciences and Psychiatry, Hacettepe University, Ankara, Türkiye.
Noro Psikiyatr Ars. 2024 Jul 20;62(3):216-219. doi: 10.29399/npa.28688. eCollection 2025.
The objective of this study is to elucidate the expression pattern and alterations of NLRP2 following a single cortical spreading depolarization (CSD).
Brain tissue was obtained by cardiac perfusion at the 15th minute after CSD in optogenetically inducible Thy1-ChR2-YFP (n=3) mice. NLRP2 expression was examined in the obtained brain tissue sections by immunofluorescence staining and compared with NLRP2 expression pattern in the sections obtained from the control group (n=3) and naive mice (n=1). The cellular source of NLRP2 is demonstrated by dual staining with NeuN and S100β.
Cellular immunoreactivity of NLRP2 was evident in the cortical and subcortical regions of sham and naive group brains. This immunoreactivity exhibited a notable reduction in CSD-induced brains. The the majority of cells expressing NLRP2 exhibited colocalization with NeuN, a neuronal marker, while a smaller subset demonstrated merging with S100β, an astrocyte marker.
This study revealed the decreased immunoreactivity of NLRP2 after single CSD in early period.
本研究的目的是阐明单次皮层扩散性去极化（CSD）后NLRP2的表达模式及变化。
在光遗传学诱导的Thy1-ChR2-YFP小鼠（n=3）中，于CSD后第15分钟通过心脏灌注获取脑组织。通过免疫荧光染色检测所获脑组织切片中NLRP2的表达，并与对照组（n=3）和未处理小鼠（n=1）的切片中NLRP2的表达模式进行比较。通过NeuN和S100β双重染色确定NLRP2的细胞来源。
在假手术组和未处理组小鼠大脑的皮质和皮质下区域，NLRP2的细胞免疫反应明显。这种免疫反应在CSD诱导的大脑中显著降低。大多数表达NLRP2的细胞与神经元标记物NeuN共定位，而一小部分与星形胶质细胞标记物S100β融合。
本研究揭示了单次CSD早期后NLRP2免疫反应性降低。
