鸭血清铁蛋白的放射免疫测定法。
A radioimmunoassay for duck serum ferritin.
作者信息
Díez J M, Agapito M T, Recio J M
出版信息
Rev Esp Fisiol. 1985 Dec;41(4):457-62.
PMID:4095367
Abstract
Development and optimization of a radioimmunoassay for duck serum ferritin described. 125I-labelled ferritin and rabbit anti-ferritin antibody are used together with goat anti-rabbit gamma globulin as separating agent for the bound and free fractions. The assay has a working range of up to 500 micrograms of ferritin per litre and a sample requirement of 50 microliter of serum. The assay requires a 24 h period and has a sensitivity of 10 micrograms of ferritin per litre.
摘要
本文描述了鸭血清铁蛋白放射免疫分析方法的建立与优化。使用¹²⁵I标记的铁蛋白和兔抗铁蛋白抗体，同时以山羊抗兔γ球蛋白作为结合和游离部分的分离剂。该分析方法的工作范围高达每升500微克铁蛋白，血清样本需求量为50微升。该分析需要24小时，灵敏度为每升10微克铁蛋白。
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