血清铁蛋白的放射免疫测定法。

A radioimmunoassay for serum ferritin.

作者信息

Luxton A W, Walker W H, Gauldie J, Ali A M, Pelletier C

出版信息

Clin Chem. 1977;23(4):683-9.

Abstract

We describe the development and evaluation of a serum ferritin radioimmunoassay, in which 125l-labeled ferritin and rabbit anti-ferritin antibody are used. Goat anti-rabbit gamma-globulin antibody, together with polyethylene glycol, is used as the separating reagent. The assay has a working range up to 500 mug of ferritin per litre, and a sample requirement of 75 mul of serum for assay at two dilutions. The assay requires 24 h. it has a sensitivity of 1.5 mug of ferritin per litre and a long-term precision (CV) of 13%. Reference intervals for a population of men were 18-330 mug/litre, with no marked age dependence, while those for a population of women older than 50 years were 18-200 mug/litre. Many apparently healthy women in the 20-50 year age group have much lower concentrations. Serum ferritin concentrations of less than 18 mug/litre are indicative of iron deficiency, defined as the absence of stainable iron in an aspirate of bone marrow.

摘要

我们描述了一种血清铁蛋白放射免疫测定法的开发与评估，该方法使用了¹²⁵I标记的铁蛋白和兔抗铁蛋白抗体。山羊抗兔γ球蛋白抗体与聚乙二醇一起用作分离试剂。该测定法的工作范围高达每升500微克铁蛋白，在两种稀释度下进行测定时样本需要75微升血清。该测定需要24小时。它的灵敏度为每升1.5微克铁蛋白，长期精密度（CV）为13%。男性群体的参考区间为18 - 330微克/升，无明显年龄依赖性，而50岁以上女性群体的参考区间为18 - 200微克/升。许多20 - 50岁年龄组看似健康的女性浓度要低得多。血清铁蛋白浓度低于18微克/升表明缺铁，缺铁定义为骨髓抽吸物中无可染色铁。