Chaicumpa W, Wechsathanarak Y, Tantivanich S, Plueksawan W
Southeast Asian J Trop Med Public Health. 1985 Sep;16(3):371-6.
The heat-killed, phenolized parenteral typhoid vaccine was tested in informed volunteers. Assessment for its immunogenicity was performed using Widal test and enzyme-linked immunosorbent assay (ELISA). The anti-H antibody, which is a marker of the vaccine antigenicity peaked at one month after the vaccination and appeared throughout the one year course of the study. The anti-O antibody peaked at 7th day after vaccination and lasted only for 6 months. Classes of specific antibodies were determined by ELISA using single extracted lipopolysaccharide from Salmonella typhi 0901 as antigen. The possible protective role of serum derived intestinal IgG and IgA were discussed. Based on the agglutinating antibodies, the results indicate that the heat-killed, phenolized typhoid vaccine conferred at least 6 months protective period.
对经热灭活、酚化处理的肠道伤寒疫苗在知情志愿者身上进行了测试。使用肥达试验和酶联免疫吸附测定（ELISA）对其免疫原性进行评估。抗-H抗体作为疫苗抗原性的标志物，在接种疫苗后1个月达到峰值，并在整个为期1年的研究过程中都有出现。抗-O抗体在接种疫苗后第7天达到峰值，仅持续6个月。使用从伤寒沙门氏菌0901中提取的单一脂多糖作为抗原，通过ELISA确定特异性抗体类别。讨论了血清来源的肠道IgG和IgA可能的保护作用。基于凝集抗体，结果表明经热灭活、酚化处理的伤寒疫苗可提供至少6个月的保护期。
