Nardiello S, Pizzella T, Russo M, Galanti B
J Clin Microbiol. 1984 Oct;20(4):718-21. doi: 10.1128/jcm.20.4.718-721.1984.
Serum samples from 22 patients with proven typhoid fever, 60 febrile nontyphoidal patients, and 120 healthy subjects were tested for immunoglobulin A (IgA), IgG, and IgM anti-Salmonella typhi lipopolysaccharide antibodies by an enzyme-linked immunosorbent assay. The levels of all three classes of immunoglobulin anti-lipopolysaccharide were higher in typhoid patients than in controls; the test for IgM anti-lipopolysaccharide gave the best discrimination between typhoid and nontyphoidal sera. The absorbance values obtained with the enzyme-linked immunosorbent assay for IgM anti-lipopolysaccharide were highly correlated to the titers of anti-O agglutinins. However, the enzyme-linked immunosorbent assay was much more specific than the Widal test, and hence it could be a useful tool for the serological diagnosis of typhoid fever with a single blood sample.
采用酶联免疫吸附测定法，对22例确诊为伤寒热的患者、60例发热的非伤寒患者以及120名健康受试者的血清样本进行免疫球蛋白A（IgA）、免疫球蛋白G（IgG）和免疫球蛋白M（IgM）抗伤寒沙门氏菌脂多糖抗体检测。伤寒患者体内所有三类抗脂多糖免疫球蛋白的水平均高于对照组；IgM抗脂多糖检测在区分伤寒血清和非伤寒血清方面表现最佳。通过酶联免疫吸附测定法获得的IgM抗脂多糖吸光度值与抗O凝集素滴度高度相关。然而，酶联免疫吸附测定法比肥达氏试验更具特异性，因此它可能是一种利用单一血样进行伤寒热血清学诊断的有用工具。
