Nardiello S, Pizzella T, Migliaresi S, Galanti B
Boll Ist Sieroter Milan. 1985;64(2):155-9.
Some characteristics of an enzyme-linked immunosorbent assay for the determination of immunoglobulin M anti-Salmonella lipopolysaccharide antibodies have been assessed. Immunoglobulin M anti-lipopolysaccharide antibodies in serum samples from most patients with typhoid fever cross-reacted with S. typhimurium lipopolysaccharide. However, absorbance values with S. typhi lipopolysaccharide were always higher than with the heterologous antigens. Serum samples from two patients with S. paratyphi B infection failed to show significant immunoglobulin M binding to S. typhi lipopolysaccharide. Serum samples from six rheumatoid arthritis patients were negative in this immunoenzymatic assay with S. typhi or S. typhimurium lipopolysaccharide as coating antigen. Finally, interference by specific immunoglobulin G in the immunoglobulin M anti-S. typhi lipopolysaccharide antibodies determination was excluded.
已对一种用于测定免疫球蛋白M抗沙门氏菌脂多糖抗体的酶联免疫吸附测定法的一些特性进行了评估。大多数伤寒热患者血清样本中的免疫球蛋白M抗脂多糖抗体与鼠伤寒沙门氏菌脂多糖发生交叉反应。然而，伤寒杆菌脂多糖的吸光度值总是高于异源抗原。两名副伤寒乙感染患者的血清样本未显示出免疫球蛋白M与伤寒杆菌脂多糖有显著结合。六名类风湿性关节炎患者的血清样本在以伤寒杆菌或鼠伤寒沙门氏菌脂多糖作为包被抗原的这种免疫酶测定中呈阴性。最后，排除了特异性免疫球蛋白G在免疫球蛋白M抗伤寒杆菌脂多糖抗体测定中的干扰。
