Khan N I, Desowitz R S
Southeast Asian J Trop Med Public Health. 1985 Sep;16(3):377-80.
Counterimmunoelectrophoresis (CIE) using cell-free extracts of Leishmania donovani promastigotes and Trypanosoma brucei as antigens and indirect haemagglutination (IHA) using commercially prepared reagents were compared for their diagnostic efficacy and applicability to immunoepidemiological studies in an area of Bangladesh where kala-azar is present. The CIE was positive for all parasitologically confirmed cases whereas the IHA positivity was only 60%. The T. brucei antigen was equally as good, if not better, than the L. donovani antigen for CIE. The CIE test was negative for all of 34 apparently healthy villagers. For this same group of individuals, 10 (29%) were low titre-IHA positive. The findings suggest that CIE is the more reliable diagnostic test but both methods should be employed for immunoepidemiological investigations.
在孟加拉国存在黑热病的一个地区，比较了以杜氏利什曼原虫前鞭毛体和布氏锥虫的无细胞提取物为抗原的对流免疫电泳（CIE）以及使用市售试剂的间接血凝试验（IHA）在诊断效力和免疫流行病学研究适用性方面的情况。CIE对所有经寄生虫学确诊的病例均呈阳性，而IHA阳性率仅为60%。对于CIE而言，布氏锥虫抗原即便不比杜氏利什曼原虫抗原更好，也同样良好。CIE检测对34名表面健康的村民全部呈阴性。对于同一组个体，10人（29%）IHA呈低滴度阳性。研究结果表明，CIE是更可靠的诊断检测方法，但两种方法都应用于免疫流行病学调查。
