Murakoshi M, Osamura Y, Watanabe K
Tokai J Exp Clin Med. 1985 Apr;10(1):5-12.
Ultrastructural alterations of 4-aminopyrazolopyrimidine (4-APP)-induced fatty liver were studied. Light microscopically, liver sections after 3 to 5 days of 4-APP administration demonstrated more extensive fatty changes and highly osmiophilic lipid droplets were mainly observed in the portal zones of the lobules. The most striking ultrastructural alterations were seen in the Golgi complexes and rough endoplasmic reticulum. Golgi complexes were markedly dilated and osmiophilic vesicles were noted near the Golgi complexes. In the rough endoplasmic reticulum, dilatation of cisternae, loss of the parallel arrangement and dilated vesicles, most of which contained diminished numbers of ribosomes, were also seen. In addition, degranulation of rough endoplasmic reticulum was observed. However, no remarkable changes were noted in the mitochondria. Based on these findings, the functional state of the organellae concerned was discussed.
研究了4-氨基吡唑并嘧啶（4-APP）诱导的脂肪肝的超微结构改变。光学显微镜下，给予4-APP 3至5天后的肝脏切片显示出更广泛的脂肪变化，并且在小叶的门管区主要观察到高嗜锇性脂滴。最显著的超微结构改变见于高尔基体和粗面内质网。高尔基体明显扩张，在高尔基体附近可见嗜锇性小泡。在粗面内质网中，也可见到池扩张、平行排列丧失以及扩张的小泡，其中大多数小泡所含核糖体数量减少。此外，还观察到粗面内质网脱颗粒。然而，线粒体未见明显变化。基于这些发现，对相关细胞器的功能状态进行了讨论。
