Rashad Mariam, Elsebaei Fawzi, Sheribah Zeinab Awad, Walash Mohamed Ibrahim
Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura University, Mansoura, 35516, Egypt.
J Fluoresc. 2025 Sep 17. doi: 10.1007/s10895-025-04545-y.
A novel, simple, and highly sensitive spectrofluorometric method was developed for the quantification of Tamsulosin (TAM) in methanol, utilizing silver nanoparticles (AgNPs) as fluorescence enhancers. The AgNPs formed colloidal dispersions with particle sizes ranging between 5 and 50 nm, enhancing TAM's native fluorescence through a catalytic interaction. Fluorescence emission was measured at 315 nm after excitation at 285 nm, and the method exhibited linearity in the range of 100-500 ng/ml. The method was successfully applied to the assay of two commercial TAM capsule formulations obtained from a local pharmacy. Validation was carried out in accordance with International Council for Harmonisation (ICH) guidelines, confirming the method's accuracy, precision, and robustness. Furthermore, the ecological impact of the proposed method was evaluated using greenness assessment tools, including AGREE and the Analytical Eco-Scale, demonstrating its sustainability in terms of solvent use, chemical consumption, energy efficiency, and waste reduction.
开发了一种新颖、简单且高度灵敏的荧光分光光度法，用于定量测定甲醇中的坦索罗辛（TAM），该方法利用银纳米颗粒（AgNPs）作为荧光增强剂。所形成的AgNPs胶体分散体的粒径在5至50纳米之间，通过催化相互作用增强了TAM的固有荧光。在285纳米激发后，于315纳米处测量荧光发射，该方法在100 - 500纳克/毫升范围内呈线性。该方法成功应用于从当地药房获得的两种市售TAM胶囊制剂的测定。按照国际协调会议（ICH）指南进行了验证，证实了该方法的准确性、精密度和稳健性。此外，使用包括AGREE和分析生态量表在内的绿色度评估工具对所提出方法的生态影响进行了评估，证明了其在溶剂使用、化学消耗、能源效率和减少废物方面的可持续性。
