Ou Jiahui, Liu Bingchen, Yu Yi, He Yingchun, Gao Yuyu, Chen Lingli, Chen Xia, Tao Huai
School of Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China.
Hunan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China.
Front Oncol. 2025 Sep 1;15:1611007. doi: 10.3389/fonc.2025.1611007. eCollection 2025.
RNA methylation modifications are widespread in eukaryotes and prokaryotes, with N6-methyladenosine (m6A) methylation being the most prevalent internal modification in eukaryotic mRNA and having become a prominent focus of tumor research in recent years. Up to now, substantial evidence has suggested that the dysregulated RNA demethylase ALKBH5 can interact with m6A reader proteins to modulate a wide range of mRNA biological progress, including mRNA shearing, export, metabolism, and stability, ultimately influencing tumorigenesis and development. To deeply understand the regulatory roles of ALKBH5 and reader proteins in tumor progression, this review aims to summarize the structures of ALKBH5 and reader proteins, as well as their cooperative regulatory mechanisms that affect the occurrence and development of tumors originating from different systems. Furthermore, the potential applications of targeting ALKBH5 and reader proteins in antitumor drug development are summarized, hoping to provide a strong basis for advancing antineoplastic research in the future.
RNA甲基化修饰在真核生物和原核生物中广泛存在，其中N6-甲基腺苷（m6A）甲基化是真核生物mRNA中最普遍的内部修饰，并且近年来已成为肿瘤研究的一个突出焦点。到目前为止，大量证据表明，失调的RNA去甲基化酶ALKBH5可以与m6A阅读蛋白相互作用，调节广泛的mRNA生物学进程，包括mRNA剪接、输出、代谢和稳定性，最终影响肿瘤的发生和发展。为了深入了解ALKBH5和阅读蛋白在肿瘤进展中的调控作用，本综述旨在总结ALKBH5和阅读蛋白的结构，以及它们影响不同系统来源肿瘤发生和发展的协同调控机制。此外，还总结了靶向ALKBH5和阅读蛋白在抗肿瘤药物开发中的潜在应用，希望为未来推进抗肿瘤研究提供有力依据。
