Moradi Mojtaba, Ghanbari Elham, Rashidi Zahra, Moradi Saman, Shakeri Ali, Faramarzi Azita
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Int J Reprod Biomed. 2025 Aug 27;23(7):545-558. doi: 10.18502/ijrm.v23i7.19482. eCollection 2025 Jul.
Cadmium (Cd) is a hazardous heavy metal, and its exposure can lead to a range of health issues, including significant adverse effects on reproductive health in animals and humans. Recently, there has been increasing recognition of the antioxidant benefits of extract (CSE).
To evaluate the therapeutic potential of CSE in mitigating testicular injury and spermatogenesis impairment induced by Cd.
In this experimental study, 40 adult male BALB/c mice (8-12 wk, 30 5 gr) were randomly divided into 4 groups (n = 10/each): control, Cd (0.35 mg/kg), CSE (100 mg/kg), and CSE+Cd (100 mg/kg + 0.35 mg/kg). Adult male mice were intraperitoneally injected for one cycle of spermatogenesis (35 days). Sperm parameters, sperm DNA integrity, testicular histopathology status, testosterone hormone level, and testicular levels of malondialdehyde, nitric oxide, and total antioxidant capacity were assessed.
CSE restored spermatogenesis by improving sperm count, motility, viability, morphology, and chromatin integrity (p 0.01). Testosterone levels and the histopathology of the testes also showed significant improvement in the CSE-administrated groups (p 0.001). More notably, Cd administration significantly induced oxidative stress in testicular tissue (p 0.001). Also, CSE restored antioxidant status by enhancing total antioxidant capacity levels and ameliorating nitric oxide and malondialdehyde levels (p 0.001).
Administering CSE could potentially enhance testis function and sperm parameters against chronic Cd exposure-induced reproductive toxicity, likely due to improving testosterone secretion and its antioxidant properties.
镉（Cd）是一种有害重金属，接触镉会导致一系列健康问题，包括对动物和人类生殖健康产生重大不利影响。最近，人们越来越认识到[提取物名称未给出]提取物（CSE）的抗氧化益处。
评估CSE在减轻镉诱导的睾丸损伤和精子发生障碍方面的治疗潜力。
在本实验研究中，40只成年雄性BALB/c小鼠（8 - 12周龄，体重30±5克）被随机分为4组（每组n = 10）：对照组、镉组（0.35毫克/千克）、CSE组（100毫克/千克）和CSE + 镉组（100毫克/千克 + 0.35毫克/千克）。成年雄性小鼠进行一个精子发生周期（35天）的腹腔注射。评估精子参数、精子DNA完整性、睾丸组织病理学状态、睾酮激素水平以及睾丸中丙二醛、一氧化氮和总抗氧化能力水平。
CSE通过改善精子数量、活力、存活率、形态和染色质完整性恢复了精子发生（p < 0.01）。在给予CSE的组中，睾酮水平和睾丸组织病理学也显示出显著改善（p < 0.001）。更值得注意的是，给予镉显著诱导了睾丸组织中的氧化应激（p < 0.001）。此外，CSE通过提高总抗氧化能力水平以及改善一氧化氮和丙二醛水平恢复了抗氧化状态（p < 0.001）。
给予CSE可能会增强睾丸功能和精子参数，以对抗慢性镉暴露诱导的生殖毒性，这可能是由于改善了睾酮分泌及其抗氧化特性。
