Szabó András, Péter Mária, Balogh Gábor, Török Zsolt, Kóta Zoltán, Vígh László, Ali Omeralfaroug, Timár Botond, Kövér György, Nagy Tibor, Olasz Ferenc, Bóta Brigitta, Petneházy Örs, Gömbös Patrik, Agyarko Edward, Thi Nguyen Anh, Varga-Visi Éva, Kovács Melinda
Agribiotechnology and Precision Breeding for Food Security National Laboratory, Department of Physiology and Animal Health, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Kaposvár 7400, Hungary.
HUN-REN-MATE Mycotoxins in the Food Chain Research Group, Kaposvár 7400, Hungary.
J Agric Food Chem. 2025 Oct 1;73(39):24975-24996. doi: 10.1021/acs.jafc.5c05715. Epub 2025 Sep 17.
Fumonisin B1 was intraperitoneally administered at 2.1 μg/animal/day (1×) and 5 and 10 times above (5× and 10×) to male rats for 5 days ( = 8/group, = 32). Bodyweight gain decreased in all FB1-treated groups after 3 days, while bodyweight decreased (10×) after 5 days. Feed intake and liver weight decreased (5×, 10×). Clinical chemistry indicated increased total protein, albumin, cholesterol, AST, ALT, and gamma-GT (10×). Histopathology revealed apoptosis, mitosis, hydropic change, mild steatosis, and pericentral glycogen depletion (10×). Shotgun lipidomics showed an increase in sphingosine at 10×, with a dose-dependent depletion of longer-chain ceramide and sphingomyelin molecular species. GM3 ganglioside and free and esterified cholesterol increased linearly with FB1 dose. Arachidonic acid (AA)-containing species of ether-type phosphatidylcholine and phosphatidylethanolamine (PE) displayed a linear dose response; those in diacyl PE and phosphatidylinositol followed logarithmic models. Transcriptomics revealed downregulation of steroid metabolism, while the NF-kB, sphingolipid, PI3K-Akt-PTEN, and TNF signaling pathways were upregulated, critically beyond 5×.
伏马菌素B1以2.1μg/动物/天（1倍剂量）、5倍剂量和10倍剂量腹腔注射给雄性大鼠，持续5天（每组8只，共32只）。3天后，所有伏马菌素B1处理组的体重增加均减少，而5天后体重下降（10倍剂量组）。采食量和肝脏重量下降（5倍剂量组、10倍剂量组）。临床化学指标显示总蛋白、白蛋白、胆固醇、谷草转氨酶、谷丙转氨酶和γ-谷氨酰转肽酶升高（10倍剂量组）。组织病理学显示凋亡、有丝分裂、水样变性、轻度脂肪变性和中央周围糖原耗竭（10倍剂量组）。鸟枪法脂质组学显示10倍剂量组鞘氨醇增加，长链神经酰胺和鞘磷脂分子种类呈剂量依赖性减少。GM3神经节苷脂以及游离和酯化胆固醇随伏马菌素B1剂量呈线性增加。含花生四烯酸（AA）的醚型磷脂酰胆碱和磷脂酰乙醇胺（PE）种类呈现线性剂量反应；二酰基PE和磷脂酰肌醇中的种类遵循对数模型。转录组学显示类固醇代谢下调，而NF-κB、鞘脂、PI3K-Akt-PTEN和TNF信号通路上调，在5倍剂量以上时变化显著。
