Terao J, Magarian R A, Brueggemann G, King M M
Anal Biochem. 1985 Dec;151(2):445-54. doi: 10.1016/0003-2697(85)90203-9.
Butylated hydroxytoluene (BHT) is a phenolic antioxidant which is widely used in foods and has been shown to inhibit chemical carcinogenesis in the mammary gland induced by 7,12-dimethylbenz(a)anthracene. However, its mechanism of action as a tumor inhibitor is unclear. The purpose of this work was first to develop a method for extracting and quantitating BHT and then to determine the amounts that accumulate in the tissues and serum of rats as a starting point for looking at mechanistic possibilities in the inhibition of mammary carcinogenesis. Methodology of extracting BHT from rat tissues and serum was developed using a modified lipid extraction procedure. The sensitive nature of reverse-phase high-performance liquid chromatography proved useful in detecting and quantifying BHT after its extraction from biological tissues. All tissues were taken from animals consuming semipurified diets with and without 0.3% BHT for various periods of time (weeks). BHT was found in much higher levels in mammary tissue than in the liver and serum of rats. The lipid content in mammary tissue appears to be predictive of the amount of BHT found in this tissue, presumably because of the lipophilic character of the antioxidant.
丁基羟基甲苯（BHT）是一种酚类抗氧化剂，广泛应用于食品中，并且已被证明能够抑制由7,12 - 二甲基苯并(a)蒽诱导的乳腺化学致癌作用。然而，其作为肿瘤抑制剂的作用机制尚不清楚。这项工作的目的首先是开发一种提取和定量BHT的方法，然后确定大鼠组织和血清中积累的BHT量，以此作为研究抑制乳腺致癌作用机制可能性的起点。采用改良的脂质提取程序开发了从大鼠组织和血清中提取BHT的方法。反相高效液相色谱的灵敏特性被证明在从生物组织中提取BHT后检测和定量BHT方面很有用。所有组织均取自食用含0.3%BHT和不含0.3%BHT的半纯化日粮不同时间段（周）的动物。在大鼠的乳腺组织中发现BHT的含量远高于肝脏和血清中的含量。乳腺组织中的脂质含量似乎可以预测该组织中BHT的含量，这可能是由于抗氧化剂的亲脂性所致。
