Dagsuyu Eda, Kies Paige, Karau Melissa J, Patel Robin, Beyenal Haluk
School of Chemical Engineering and Bioengineering, Voiland College of Engineering and Architecture, Washington State University, Pullman, Washington, USA.
Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, Istanbul, Türkiye.
Microbiol Spectr. 2025 Sep 18:e0176225. doi: 10.1128/spectrum.01762-25.
Biofilms hinder wound infection healing, making wound infections a healthcare challenge for which innovative treatments are needed. A 1.77 cm electrochemical bandage (e-bandage), which generates the biocide hypochlorous acid (HOCl), has shown promising anti-biofilm activity , , and . The e-bandage is a three-electrode system operationalized by a hydrogel. In addition to providing a conductive electrolyte for the electrodes, the hydrogel provides a protective barrier for the wound. How different hydrogels impact e-bandage functionality is not completely defined. Here, the activity of seven clinically available hydrogels (xanthan gum, 3M, Duoderm, Prontosan, Purilon, Skintegrity, and Solosite) was evaluated with 1.77 cm HOCl-generating e-bandages using a potentiostat with a multiplexer in a membrane biofilm model against methicillin-resistant IDRL-6169 and ATCC-17978. When applied with polarized HOCl-producing e-bandages, all evaluated hydrogels except Solosite yielded ≥5.5 logCFU/cm reductions in bacterial quantities after 6 h of treatment, with most being below the detection limit at that time point. Prontosan exhibited antibacterial activity independent of e-bandage. Results of this study inform the selection of clinically available hydrogels that may be suitable for use with a 1.77 cm HOCl-producing e-bandage.
In this study, it was shown that some clinically available hydrogels, combined with a 1.77 cm HOCl-generating electrochemical bandage, deliver time-dependent antimicrobial activity against methicillin-resistant and biofilms, offering a potential approach to treat wound infections, including those caused by antimicrobial-resistant bacteria.
生物膜阻碍伤口感染愈合，使伤口感染成为一个需要创新治疗方法的医疗挑战。一种1.77厘米的电化学绷带（电子绷带），可产生杀菌剂次氯酸（HOCl），已显示出有前景的抗生物膜活性 ， ， 和 。该电子绷带是一个由水凝胶操作的三电极系统。除了为电极提供导电电解质外，水凝胶还为伤口提供保护屏障。不同水凝胶如何影响电子绷带功能尚未完全明确。在此，使用带有多路复用器的恒电位仪，在膜生物膜模型中，针对耐甲氧西林的IDRL - 6169和ATCC - 17978，用1.77厘米产生HOCl的电子绷带评估了七种临床可用水凝胶（黄原胶、3M、杜德棉、普朗托散、普立龙、皮肤完整性和索洛西特）的活性。当与产生极化HOCl的电子绷带一起应用时，除索洛西特外，所有评估的水凝胶在治疗6小时后细菌数量减少≥5.5 logCFU/cm，大多数在该时间点低于检测限。普朗托散表现出与电子绷带无关的抗菌活性。本研究结果为选择可能适用于1.77厘米产生HOCl的电子绷带的临床可用水凝胶提供了依据。
在本研究中，表明一些临床可用水凝胶与1.77厘米产生HOCl的电化学绷带相结合，对耐甲氧西林的 和生物膜具有时间依赖性抗菌活性，为治疗伤口感染，包括由耐药细菌引起的感染，提供了一种潜在方法。
