Sanoie Maryam, Teymoori Farshad, Abooshahab Raziyeh, Akbarzadeh Mahdi, Asghari Golaleh, Yuzbashian Emad, Hedayati Mehdi, Khalaj Alireza, Zarkesh Maryam
Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Molecular Biology, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Endocrinol Diabetes Metab. 2025 Sep;8(5):e70080. doi: 10.1002/edm2.70080.
This study aimed to investigate the relationship between plasma fatty acids (FAs), FA-derived factors and PPARγ expression in visceral and subcutaneous adipose tissues (VAT and SAT) of obese and nonobese adults.
This cross-sectional study involved 167 adults aged 19 to 65. Samples of VAT and SAT were obtained during elective abdominal surgeries. Participants were divided into two groups: nonobese (BMI < 30 kg/m) and obese (BMI ≥ 30 kg/m). Anthropometric and biochemical measurements were taken, and plasma fatty acids (FAs) were analysed using gas chromatography flame ionisation detection (GC/FID). PPARγ mRNA levels were measured through real-time RT-qPCR.
Obese individuals had higher PPARγ gene expression in both VAT and SAT compared to nonobese participants (p < 0.001). Eighteen FFAs and three new FA-derived factors were identified in both groups, accounting for 69% of the variance in nonobese individuals and 71% in obese individuals. After adjusting for confounding factors, saturated FA (SFA) was associated with PPARγ expression in the SAT of the nonobese group (β = -0.12, p = 0.019). Additionally, total FAs (β = -0.02, p = 0.017), SFA (β = -0.06, p = 0.048), monounsaturated FA (MUFA) (β = -0.08, p = 0.020), polyunsaturated FA (PUFA) (β = -0.03, p = 0.039) and omega-6 FA (β = -0.03, p = 0.040) were associated with VAT PPARγ expression among obese individuals. Conversely, an inverse correlation was observed between factor I of FAs and SAT PPARγ expression in nonobese individuals (β = -0.15; p = 0.027).
These findings suggest that alterations in plasma FA profiles are associated with PPARγ gene expression, particularly in obese individuals. This fact highlights the potential role of dietary FAs in metabolic regulation and health issues related to obesity.
本研究旨在调查肥胖和非肥胖成年人内脏及皮下脂肪组织（VAT和SAT）中血浆脂肪酸（FAs）、FA衍生因子与PPARγ表达之间的关系。
这项横断面研究纳入了167名年龄在19至65岁之间的成年人。在择期腹部手术期间获取VAT和SAT样本。参与者被分为两组：非肥胖组（BMI < 30 kg/m²）和肥胖组（BMI ≥ 30 kg/m²）。进行人体测量和生化检测，并使用气相色谱火焰离子化检测（GC/FID）分析血浆脂肪酸（FAs）。通过实时RT-qPCR测量PPARγ mRNA水平。
与非肥胖参与者相比，肥胖个体的VAT和SAT中PPARγ基因表达均更高（p < 0.001）。两组中均鉴定出18种游离脂肪酸（FFAs）和3种新的FA衍生因子，它们在非肥胖个体中解释了69%的变异，在肥胖个体中解释了71%的变异。在调整混杂因素后，饱和脂肪酸（SFA）与非肥胖组SAT中的PPARγ表达相关（β = -0.12，p = 0.019）。此外，总脂肪酸（β = -0.02，p = 0.017）、SFA（β = -0.06，p = 0.048）、单不饱和脂肪酸（MUFA）（β = -0.08，p = 0.020）、多不饱和脂肪酸（PUFA）（β = -0.03，p = 0.039）和ω-6脂肪酸（β = -0.03，p = 0.040）与肥胖个体VAT中的PPARγ表达相关。相反，在非肥胖个体中观察到FAs的因子I与SAT中的PPARγ表达呈负相关（β = -0.15；p = 0.027）。
这些发现表明血浆FA谱的改变与PPARγ基因表达相关，尤其是在肥胖个体中。这一事实凸显了膳食脂肪酸在代谢调节以及与肥胖相关的健康问题中的潜在作用。
