Timko J, Zelinka J
J Antibiot (Tokyo). 1977 Aug;30(8):644-8. doi: 10.7164/antibiotics.30.644.
Nucleic acids from Streptomyces griseus 178 were isolated during cultivation. After their fractionation on a column of methylated serum albumin adsorbed on Kieselguhr, the 16 S and 23 S RNA were isolated. To characterize RNAs their sedimentation coefficients, Tm and nucleotide composition were determined. During cultivation of S. griseus 178 rRNA level reaches two maximum peaks and the production of streptomycin influences nucleic aicds of the producer organism.
在链霉菌178的培养过程中分离出核酸。将其在吸附于硅胶的甲基化血清白蛋白柱上进行分级分离后,分离出16S和23S RNA。为了表征RNA,测定了它们的沉降系数、解链温度和核苷酸组成。在链霉菌178的培养过程中,rRNA水平达到两个最大值峰,链霉素的产生会影响产生菌的核酸。