Španko Michal, Pfeiferová Lucie, Krejčí Eliška Drobná, Kolář Michal, Dundr Pavel, Valach Jaroslav, Smetana Karel, Lacina Lukáš
Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Department of Stomatology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Histochem Cell Biol. 2025 Sep 19;163(1):93. doi: 10.1007/s00418-025-02419-9.
Cancer-associated fibroblasts (CAFs) represent an important component of the cancer ecosystem, influencing the broad scale of biological properties of tumour cells, including the capacity for metastasis formation. An important CAF subpopulation, known as myCAFs, typically expresses α-smooth muscle actin. Transcriptomic analysis demonstrated that activated fibroblasts isolated from various pathological tissues also express the ACTG2 gene encoding γ-smooth muscle actin. This was further validated by immunocytochemistry. Using the scratch test in vitro, it was possible to demonstrate that γ-smooth muscle actin may be associated with the epithelial-mesenchymal transition, which was also shown by transcriptomic analysis. The presence of γ-smooth muscle actin-positive fibroblasts in histopathological sections of human tumours verified the expression of this protein as a new potential marker of CAFs.
癌症相关成纤维细胞（CAFs）是癌症生态系统的重要组成部分，影响肿瘤细胞广泛的生物学特性，包括转移形成能力。一个重要的CAF亚群，称为肌成纤维细胞（myCAFs），通常表达α-平滑肌肌动蛋白。转录组分析表明，从各种病理组织中分离出的活化成纤维细胞也表达编码γ-平滑肌肌动蛋白的ACTG2基因。这通过免疫细胞化学进一步得到验证。使用体外划痕试验，有可能证明γ-平滑肌肌动蛋白可能与上皮-间质转化有关，转录组分析也表明了这一点。人肿瘤组织病理学切片中γ-平滑肌肌动蛋白阳性成纤维细胞的存在证实了该蛋白作为CAFs新的潜在标志物的表达。
