Ghayasuddin M, Shulman S
Immunology. 1970 Aug;19(2):225-37.
Rabbit thyroglobulin was enzymatically digested with trypsin, and fragments were isolated through gel filtration. A 10-hour enzyme digest was studied in detail. Upon examination in a synthetic-boundary cell in the ultracentrifuge it revealed a predominantly slow-sedimenting component with a 1 S value. The various fragments of this digest were tested against three different antisera, i.e. heteroantisera to whole thyroglobulin, and to fragments of thyroglobulin, and an isoantiserum, containing autoantibodies, to whole thyroglobulin. The thyroglobulin fragments reacted strongly with the heteroantisera, especially with the antiserum to thyroglobulin fragments, but the same fragments reacted very poorly with the autoantibodies. The former antiserum revealed six lines of precipitation whereas the latter antiserum showed only two lines. These observations supported the hypothesis that a lesser number of antigenic determinants on the thyroglobulin molecule were active against autoantibodies as compared to the heteroantibodies.
用胰蛋白酶对兔甲状腺球蛋白进行酶解，并通过凝胶过滤分离片段。对10小时的酶解产物进行了详细研究。在超速离心机的合成边界池中进行检测时，发现其主要是一个沉降缓慢的组分，沉降系数为1S。将该酶解产物的各种片段与三种不同的抗血清进行检测，即针对全甲状腺球蛋白的异种抗血清、针对甲状腺球蛋白片段的异种抗血清，以及含有针对全甲状腺球蛋白自身抗体的同种抗血清。甲状腺球蛋白片段与异种抗血清反应强烈，尤其是与针对甲状腺球蛋白片段的抗血清反应强烈，但相同的片段与自身抗体反应很差。前一种抗血清显示出六条沉淀线，而后一种抗血清仅显示出两条沉淀线。这些观察结果支持了这样一种假说，即与异种抗体相比，甲状腺球蛋白分子上针对自身抗体的抗原决定簇数量较少。
