Stylos W A, Rose N R
Clin Exp Immunol. 1977 Feb;27(2):245-53.
Purified human thyroglobulin (Tg) was hydrolysed by pepsin. After completion of hydrolysis the pepsin hydrolysate was passed through a Sephadex G-200 column to remove undigested Tg. Further isolation of the enzymatic fragments was effected by passage through a Sephadex G-75 column. Two discrete fragments, termed pep I and pep II, were separated. The two fragments had sedimentation coefficients of 1-0 and 0-6, respectively. These fragments retained antigenic determinants reactive with both hetero-and auto-antibodies to Tg. The larger fragment, pep I, possessed all antigenic determinants to intact Tg while pep II lacked some determinants. Neither fragment contained novel determinants resulting from proteolytic degradation.
纯化的人甲状腺球蛋白（Tg）用胃蛋白酶水解。水解完成后，胃蛋白酶水解产物通过葡聚糖G - 200柱以去除未消化的Tg。通过葡聚糖G - 75柱进一步分离酶解片段。分离出两个离散的片段，分别称为肽段I和肽段II。这两个片段的沉降系数分别为1.0和0.6。这些片段保留了与Tg的异源和自身抗体反应的抗原决定簇。较大的片段肽段I具有完整Tg的所有抗原决定簇，而肽段II缺乏一些决定簇。两个片段均不包含蛋白水解降解产生的新决定簇。
