Wang Ziqing, Yang Tao, Lupo Federico, Behrens Lara-Marie, Janssen Anika, Coffelt Seth B, Prinz Immo, Sandrock Inga, Ravens Sarina
Institute of Immunology, Hannover Medical School, Hannover, Germany.
School of Cancer Sciences, University of Glasgow, Glasgow, UK.
Eur J Immunol. 2025 Sep;55(9):e70061. doi: 10.1002/eji.70061.
γδ T cells are unconventional T cells that group into different subsets based on the usage of variable γδ T cell receptor (TCR) gene segments, body location, and functionality. γδ T cells that secrete the proinflammatory cytokine interleukin 17 (IL-17) predominantly express a Vγ4 or Vγ6 γδ TCR. The biology and the importance of the γδ TCR of IL-17-producing γδ T cells are not well understood. Here, we investigated the IL-17 production capability of γδ T cells in mice deficient for Vγ4 and Vγ6 γδ TCRs using flow cytometry, TCR-seq, and single-cell transcriptomics. Our data show that Vγ1 T cells only partially compensate for the loss of IL-17 Vγ4 and Vγ6 T cell subsets in lymphoid and nonlymphoid tissues. They develop pre- and postnatally and were predominantly detectable in their physiological body habitats. Collectively, the data underscore the nonredundant roles of Vγ4⁺ and Vγ6⁺ subsets in IL-17-mediated immunity.
γδ T细胞是非常规T细胞，根据可变γδ T细胞受体（TCR）基因片段的使用情况、身体位置和功能可分为不同亚群。分泌促炎细胞因子白细胞介素17（IL-17）的γδ T细胞主要表达Vγ4或Vγ6 γδ TCR。产生IL-17的γδ T细胞的γδ TCR的生物学特性及其重要性尚未完全了解。在此，我们使用流式细胞术、TCR测序和单细胞转录组学研究了缺乏Vγ4和Vγ6 γδ TCR的小鼠中γδ T细胞产生IL-17的能力。我们的数据表明，Vγ1 T细胞只能部分补偿淋巴组织和非淋巴组织中IL-17 Vγ4和Vγ6 T细胞亚群的缺失。它们在出生前和出生后发育，并且主要在其生理身体栖息地中被检测到。总体而言，这些数据强调了Vγ4⁺和Vγ6⁺亚群在IL-17介导的免疫中的非冗余作用。
