Ma Danni, Cai Fuman, Zhang Ting
School of Nursing, Wenzhou Medical University, Wenzhou, 325035, China.
Eur J Clin Microbiol Infect Dis. 2025 Sep 20. doi: 10.1007/s10096-025-05250-1.
Facing challenges in the fields of microbial detection and antimicrobial resistance (AMR) monitoring, the scientific community is opening new research avenues with the help of cutting-edge technologies such as molecular biology, genomics, proteomics, nanotechnology, and bioinformatics. In this review, we comprehensively collate and elaborate on revolutionary detection methods and AMR surveillance strategies that go beyond traditional microbial culture techniques. These innovative methods have not only improved the sensitivity and speed of detection but also broadened our understanding of the microbial world, providing new weapons in the fight against drug-resistant microorganisms. Through the integration and innovation of interdisciplinary approaches, we are gradually constructing a more precise, efficient, and comprehensive new paradigm for microbial detection and AMR testing.
面对微生物检测和抗菌药物耐药性（AMR）监测领域的挑战，科学界正在借助分子生物学、基因组学、蛋白质组学、纳米技术和生物信息学等前沿技术开辟新的研究途径。在本综述中，我们全面整理并阐述了超越传统微生物培养技术的革命性检测方法和AMR监测策略。这些创新方法不仅提高了检测的灵敏度和速度，还拓宽了我们对微生物世界的认识，为对抗耐药微生物提供了新武器。通过跨学科方法的整合与创新，我们正在逐步构建一个更精确、高效和全面的微生物检测及AMR测试新范式。
