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人及猪内因子与内因子I型抗体的反应

Reactions of human and hog intrinsic factors with type I antibody to intrinsic factor.

作者信息

Gullberg R

出版信息

Clin Exp Immunol. 1970 Oct;7(4):453-62.

Abstract

A simple and rapid small-scale gel filtration method was applied in studies of type I antibody to intrinsic factor using radioactive vitamin B of high specific activity and purified human and hog intrinsic factor preparations, taking into account the unsaturated B-binding capacity of the individual pernicious anaemia sera. This procedure allows the use of small amounts of reagents. Evidence was obtained for a close antigenic similarity of determinants of human and hog intrinsic factor. The use of purified intrinsic-factor preparations is important.

摘要

考虑到个别恶性贫血血清的不饱和B结合能力,采用一种简单快速的小规模凝胶过滤方法,使用高比活放射性维生素B以及纯化的人源和猪源内因子制剂,对I型内因子抗体进行研究。该方法允许使用少量试剂。已获得证据表明人源和猪源内因子的决定簇具有密切的抗原相似性。使用纯化的内因子制剂很重要。

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本文引用的文献

6
Immunologic studies with intrinsic factor in man.人体中内因子的免疫学研究。
J Clin Invest. 1963 Mar;42(3):368-82. doi: 10.1172/JCI104723.
8
A study of precipitating antibodies against human intrinsic factor.
Acta Med Scand. 1962 Oct;172:385-8. doi: 10.1111/j.0954-6820.1962.tb07170.x.
9
Electrophoresis of human gastric juice.人胃液的电泳
Nature. 1959 Dec 12;184:1848-9. doi: 10.1038/1841848a0.

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