Schade S G, Abels J, Schilling R F
J Clin Invest. 1967 Apr;46(4):615-20. doi: 10.1172/JCI105563.
Sera from a group of 79 patients with pernicious anemia were studied for the presence of antibody to intrinsic factor. Two general types of antibody activity were found, and it was possible to distinguish three groups of pernicious anemia sera on the basis of their content of these types. Type I antibody blocks the binding of radioactive vitamin B(12) to intrinsic factor when added to intrinsic factor before the B(12); it is not detected on intrinsic factor when added after B(12). This antibody blocks intrinsic factor-mediated B(12) absorption in vivo when mixed in the sequence intrinsic factor + antibody I + B(12), but not when mixed in the sequence intrinsic factor + B(12) + antibody I. Type II antibody reacts with intrinsic factor when B(12) is attached. This antibody prevents the absorption of B(12) from intrinsic factor in pernicious anemia patients when mixed in the sequence intrinsic factor + B(12) + antibody II, and is thereby distinguished from antibody I.
对一组79例恶性贫血患者的血清进行了内因子抗体检测。发现了两种一般类型的抗体活性，并且根据这些类型的含量可以区分出三组恶性贫血血清。I型抗体在放射性维生素B12与内因子结合前加入内因子时，会阻断其结合；在B12加入后加入内因子时，则检测不到该抗体。当按内因子+抗体I + B12的顺序混合时，这种抗体在体内会阻断内因子介导的B12吸收，但按内因子+ B12 +抗体I的顺序混合时则不会。II型抗体在B12附着时与内因子发生反应。当按内因子+ B12 +抗体II的顺序混合时，这种抗体可阻止恶性贫血患者从内因子中吸收B12，从而与抗体I区分开来。
