在犬类中度急性等容性血液稀释模型中，血红蛋白氧亲和力不变。
Hemoglobin Oxygen Affinity Does Not Change in the Canine Model of Moderate Acute Normovolemic Hemodilution.
作者信息
Sheng Ming, Li Weidan, You Guoxing, Wang Ying, Li Penglong, Tao Shiyu, Gao Daoyuan, Zhou Hong, Zhao Lian
机构信息
Academy of Military Science of the Chinese People's Liberation Army, Academy of Military Medical Sciences, No. 27 Taiping Road, Haidian, Beijing 100850, P. R. China.
出版信息
ACS Omega. 2025 Sep 3;10(36):41620-41629. doi: 10.1021/acsomega.5c05248. eCollection 2025 Sep 16.
OBJECTIVE
As the amount of hemoglobin (Hb) decreases during moderate acute normovolemic hemodilution (ANH), it is unclear whether the Hb-oxygen affinity (HOA) changes accordingly. Thus, this study aimed to explore changes in HOA during moderate ANH.
METHODS
A moderate ANH model of canine was established. Hemodynamic, blood gas, biochemical, oxygenation, and HOA-related parameters were measured. was obtained using three methods. One was derived from the oxygen dissociation curve (ODC) by the Bloodox-2018 analyzer, and the other two were based on blood gas analyzers.
RESULTS
With decreased Hb and hematocrit (HCT) levels during ANH, the temperature (T) and base excess (BE) decreased, while Cl increased. Compared with baseline (BL), in hemodilution 1 (HCT decreased to 75% of the BL, H1) and hemodilution 2 (HCT decreased to 50% of the BL, H2), pH and HCO decreased. Methemoglobin (MetHb) levels in H2 were significantly higher than those in BL and H1. HOA-related parameters did not change significantly in moderate ANH. Factors that increase or decrease the offset each other, leaving the unchanged in moderate ANH. The obtained based on the two blood gas analyzers was consistent, but neither was consistent with derived from the ODC.
目的
在中度急性等容性血液稀释（ANH）过程中，随着血红蛋白（Hb）含量降低，Hb与氧的亲和力（HOA）是否相应改变尚不清楚。因此，本研究旨在探讨中度ANH期间HOA的变化。
方法
建立犬中度ANH模型。测量血流动力学、血气、生化、氧合及HOA相关参数。采用三种方法获取[具体参数]。一种是通过Bloodox - 2018分析仪从氧解离曲线（ODC）得出，另外两种基于血气分析仪。
结果
ANH期间，随着Hb和血细胞比容（HCT）水平降低，体温（T）和碱剩余（BE）降低，而Cl升高。与基线（BL）相比，在血液稀释1（HCT降至BL的75%，即H1）和血液稀释2（HCT降至BL的50%，即H2）时，pH和HCO[具体数值]降低。H2中的高铁血红蛋白（MetHb）水平显著高于BL和H1。HOA相关参数在中度ANH中无显著变化。增加或降低[具体参数]的因素相互抵消，使得中度ANH中的[具体参数]保持不变。基于两种血气分析仪获得的[具体参数]一致，但均与从ODC得出的[具体参数]不一致。
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