Rizou Evgenia, Monokrousos Nikolaos, Kardami Triantafyllia, Baliota Georgia V, Rumbos Christos I, Athanassiou Christos G, Tsiropoulos Nikolaos, Ntalli Nikoletta
University Center of International Programmes of Studies, International Hellenic University, 57001 Thessaloniki, Greece.
Analytical Chemistry and Pesticides Laboratory, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, 38446 Volos, Greece.
BioTech (Basel). 2025 Sep 8;14(3):71. doi: 10.3390/biotech14030071.
Insect-derived frass is gaining attention as a circular bioeconomy product with fertilizing and pest-suppressive potential. This study investigates frass as a soil amendment for promoting beneficial nematodes and suppressing . A 40-day pot experiment on clay loam soil tested with six inputs: raw and heat-treated frass (0.5%, 1% /), fruit powder (1.6%), and an untreated control. Soil nematode communities were assessed at 5 and 40 days after application (DAA), and nematicidal activity was evaluated in vitro. Raw frass at 1% induced a rapid response from free-living nematodes at 5 DAA, with increased abundance of bacterivorous taxa such as and , alongside a higher Enrichment Index (EI), indicating short-term nutrient availability. At 40 DAA, only 1% raw frass consistently supported more cp-1 bacterivores and slightly increased Shannon diversity. Network analysis revealed more connected, modular structures in raw frass treatments, suggesting enhanced food web complexity. However, omnivore and predator effects were limited. Raw frass extracts caused over 80% paralysis of juveniles within 24 h, significantly outperforming heat-treated frass and extracts. frass moderately stimulates opportunistic nematodes and provides strong nematicidal effects, supporting its potential as a multifunctional input for sustainable soil management.
昆虫源虫粪作为一种具有施肥和抑制害虫潜力的循环生物经济产品正受到关注。本研究调查了虫粪作为土壤改良剂对促进有益线虫和抑制[未提及具体抑制对象]的作用。在黏壤土上进行了为期40天的盆栽试验，测试了六种投入物：生虫粪和热处理虫粪（0.5%、1%/）、果粉（1.6%）以及未处理的对照。在施用后5天和40天（DAA）评估土壤线虫群落，并在体外评估杀线虫活性。1%的生虫粪在施用后5天引起了自由生活线虫的快速反应，食细菌类群如[未提及具体类群]和[未提及具体类群]的丰度增加，同时富集指数（EI）更高，表明短期养分有效性。在施用后40天，只有1%的生虫粪持续支持更多的cp-1食细菌线虫，并略微增加了香农多样性。网络分析显示生虫粪处理中有更多相互连接的模块化结构，表明食物网复杂性增强。然而，杂食性和捕食性线虫的影响有限。生虫粪提取物在24小时内导致超过80%的[未提及具体线虫种类]幼虫麻痹，显著优于热处理虫粪和[未提及具体提取物]提取物。虫粪适度刺激机会性线虫并具有强大的杀线虫作用，支持其作为可持续土壤管理多功能投入物的潜力。
