Rouhová Lenka, Krejčová Gabriela, Ferreira Ana Beatriz Barletta, Maaroufi Houda Ouns, Noriega Fernando G, Barillas-Mury Carolina, Sehadová Hana, Nouzova Marcela
Institute of Entomology, Biology Centre, Czech Academy of Sciences, 370 05, České Budějovice, Czech Republic.
Faculty of Science, University of South Bohemia, 370 05, České Budějovice, Czech Republic.
Cell Tissue Res. 2025 Sep 22. doi: 10.1007/s00441-025-04012-x.
In the larvae of the mosquito Aedes aegypti, the three most important endocrine glands, the corpora allata (CA), the corpora cardiaca (CC), and the prothoracic gland (PG), together form the glandular complexes (GC). Using confocal laser scanning microscopy in combination with immunohistochemistry, in situ hybridization, ultrastructural expansion microscopy, and apoptosis studies, we were able to identify the different cell types of the GC and follow their fate during metamorphosis. Our studies revealed that the CC is not a well-defined organ but consists of individual cells randomly distributed within the GC and CA-CC complexes. Furthermore, imaging and in situ hybridization show that the CA is a compact organ composed of a single cell type. We observed that CA and CC survive during the larval-to-adult transition, while PG undergoes apoptosis and disappears within the first 24 h of adult life. This study lays the foundation for a more detailed understanding of the structure and changes in the major endocrine organs of mosquitoes, which are vectors of several important infectious diseases.
在埃及伊蚊幼虫中，三个最重要的内分泌腺，即咽侧体（CA）、心侧体（CC）和前胸腺（PG），共同构成了腺体复合体（GC）。通过将共聚焦激光扫描显微镜与免疫组织化学、原位杂交、超微结构扩展显微镜和凋亡研究相结合，我们能够识别GC的不同细胞类型，并追踪它们在变态过程中的命运。我们的研究表明，CC并非一个界限分明的器官，而是由随机分布在GC和CA-CC复合体内的单个细胞组成。此外，成像和原位杂交显示，CA是一个由单一细胞类型构成的紧密器官。我们观察到，CA和CC在幼虫到成虫的转变过程中存活下来，而PG则经历凋亡并在成虫生命的最初24小时内消失。这项研究为更详细地了解蚊子主要内分泌器官的结构和变化奠定了基础，蚊子是几种重要传染病的传播媒介。
