骆驼乳清蛋白及其胰蛋白酶水解产物对实验性旋毛虫病的预防和治疗活性评估

Evaluation of prophylactic and therapeutic activity of camel whey protein and its trypsinized hydrolysate against experimental trichinellosis.

作者信息

Abdel Salam Sara A, El-Attar Aisha, Abdelaziz Marwa, Rashed Hoda A

机构信息

Faculty of Medicine, Department of Medical Parasitology, Alexandria University, Alexandria, Egypt.

Faculty of Agriculture, Department of Dairy Science and Technology, Alexandria University, Alexandria, Egypt.

出版信息

BMC Complement Med Ther. 2025 Sep 22;25(1):331. doi: 10.1186/s12906-025-05083-7.

DOI:10.1186/s12906-025-05083-7

PMID:40983892

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12455785/

Abstract

BACKGROUND

To date, control of trichinellosis has been dependent on the conventional chemotherapeutic agent, albendazole (ALB), with its hitherto unsolved flaws, including a serious adverse profile, minor activity against muscle larva (ML) stages, and resistance development. Camel whey protein (CWP), a unique non-pharmaceutical nutraceutical, has altered the treatment trajectory of a plethora of pathological conditions. The present study aimed to evaluate the prophylactic and therapeutic profile of CWP and its tryptic hydrolysate (TWH) against experimental intestinal and muscular trichinellosis.

METHODS

CWP and TWH were characterized by free amino acids analysis using the HPLC Pico-Tag method and DPPH free radical scavenging antioxidant assay. 200 µl of five-day oral dosing of CWP and its TWH was prophylactically and therapeutically administered to mice. Parasitological, ultrastructural, biochemical, and histopathological studies were performed for assessment of their anti-trichinella activity. Preclinical data were subjected to analysis of variance and a Tukey post hoc test for pairwise comparisons.

RESULTS

The free amino acids profile and high DPPH scavenging antioxidant activity of CWP (79.2%) and TWH (82.7%) were determined. Parasitologically, TWH showed the highest prophylactic (84.0%R and 83.8%R) and therapeutic (94.6%R and 93.9%R) activity in mean worm count recovered from intestinal and muscular stages, respectively. Lesser reductions were recorded by CWP, whether prophylactically-treated (73.9%R and 72.1%R) or therapeutically-treated (84.5%R and 83.9%R) against aforementioned stages, respectively. In addition, scanning electron microscopy revealed that the most severe ultrastructural deformities were observed in TWH-treated worms. Biochemically, the prophylactic and therapeutic administration of TWH recorded the highest antioxidant serum level of reduced glutathione (GSH) that curbed the oxidant malondialdehyde (MDA). Histopathologically, TWH ameliorated the intestinal and muscular pathology compared to CWP.

CONCLUSIONS

To the best of our knowledge, this is the first study on the prophylactic and therapeutic administration of CWP and TWH against experimental trichinellosis, showing the superiority of TWH in inducing multistage activity and ameliorating the intestinal and muscular pathology.

摘要

背景

迄今为止，旋毛虫病的控制一直依赖于传统化疗药物阿苯达唑（ALB），但其存在尚未解决的缺陷，包括严重的不良反应、对肌幼虫（ML）阶段活性较低以及产生耐药性。骆驼乳清蛋白（CWP）是一种独特的非药物营养保健品，已改变了多种病理状况的治疗轨迹。本研究旨在评估CWP及其胰蛋白酶水解产物（TWH）对实验性肠道和肌肉旋毛虫病的预防和治疗效果。

方法

采用高效液相色谱Pico-Tag法和DPPH自由基清除抗氧化测定法对CWP和TWH进行游离氨基酸分析和抗氧化活性表征。将200μl的CWP及其TWH进行为期五天的口服给药，分别对小鼠进行预防性和治疗性给药。进行寄生虫学、超微结构、生化和组织病理学研究以评估其抗旋毛虫活性。对临床前数据进行方差分析和Tukey事后检验以进行两两比较。

结果

测定了CWP（79.2%）和TWH（82.7%）的游离氨基酸谱和较高的DPPH清除抗氧化活性。在寄生虫学方面，TWH在从肠道和肌肉阶段回收的平均虫数方面显示出最高的预防活性（84.0%R和83.8%R）和治疗活性（94.6%R和93.9%R）。CWP无论是在预防性治疗（73.9%R和72.1%R）还是治疗性治疗（84.5%R和83.9%R）时，对上述阶段的降低程度都较小。此外，扫描电子显微镜显示，在TWH处理的虫体中观察到最严重的超微结构畸形。在生化方面，TWH的预防性和治疗性给药记录了最高的抗氧化血清水平，即还原型谷胱甘肽（GSH），其抑制了氧化型丙二醛（MDA）。在组织病理学方面，与CWP相比，TWH改善了肠道和肌肉病理学。