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通过 DPPH 自由基清除活性测定和定量植物化学分析。

Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of .

机构信息

Centre for Drug Design Discovery and Development (C4D), SRM University, Delhi-NCR, Rajiv Gandhi Education City, Sonepat 131029, Haryana, India.

Master & Ph.D. Program in Biotechnology Industry, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan.

出版信息

Molecules. 2022 Feb 16;27(4):1326. doi: 10.3390/molecules27041326.

Abstract

The use of might be beneficial in inflammatory illnesses and can be used for a variety of health conditions. In this article, we studied the identification of antioxidants using (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical scavenging activity in , as is an important herbal plant, and every part of it has various medicinal properties such as antibacterial properties that can be used by the researchers in the development and design of various new drugs. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a popular, quick, easy, and affordable approach for the measurement of antioxidant properties that includes the use of the free radicals used for assessing the potential of substances to serve as hydrogen providers or free-radical scavengers (FRS). The technique of DPPH testing is associated with the elimination of DPPH, which would be a stabilized free radical. The free-radical DPPH interacts with an odd electron to yield a strong absorbance at 517 nm, i.e., a purple hue. An FRS antioxidant, for example, reacts to DPPH to form DPPHH, which has a lower absorbance than DPPH because of the lower amount of hydrogen. It is radical in comparison to the DPPH-H form, because it causes decolorization, or a yellow hue, as the number of electrons absorbed increases. Decolorization affects the lowering capacity significantly. As soon as the DPPH solutions are combined with the hydrogen atom source, the lower state of diphenylpicrylhydrazine is formed, shedding its violet color. To explain the processes behind the DPPH tests, as well as their applicability to () in the manufacture of metal oxide nanoparticles, in particular MgO, and their influence on antioxidants, a specimen from the test was chosen for further study. According to our findings, has antioxidant qualities and may be useful in the treatment of disorders caused by free radicals.

摘要

使用 可能对炎症性疾病有益,并且可用于多种健康状况。在本文中,我们研究了使用(DPPH)2,2-二苯基-1-苦基肼自由基清除活性来鉴定抗氧化剂,因为 是一种重要的草药植物,其各个部分都具有各种药用特性,例如抗菌特性,研究人员可以在开发和设计各种新药中使用。2,2-二苯基-1-苦基肼(DPPH)是一种流行,快速,简便且经济实惠的方法,可用于测量抗氧化特性,包括使用自由基来评估物质作为氢供体或自由基清除剂(FRS)的潜力。DPPH 测试技术与 DPPH 的消除有关,DPPH 是一种稳定的自由基。游离基 DPPH 与奇数电子相互作用,在 517nm 处产生强吸收,即紫色。例如,FRS 抗氧化剂与 DPPH 反应生成 DPPHH,由于氢的量较少,因此其吸收较低。与 DPPH-H 形式相比,它是自由基,因为它导致褪色或黄色,因为吸收的电子数增加。褪色会显着影响降低能力。一旦 DPPH 溶液与氢原子源结合,就会形成低状态的二苯苦基肼,从而失去其紫色。为了解释 DPPH 测试背后的过程以及它们在制造金属氧化物纳米粒子,特别是 MgO 中的应用,以及它们对抗氧化剂的影响,选择了测试中的一个样本进行进一步研究。根据我们的发现, 具有抗氧化特性,可用于治疗由自由基引起的疾病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e038/8878429/0cf40cb2fd4b/molecules-27-01326-g001.jpg

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