荧光抗体法核染色研究。I. 导致核荧光的非免疫因素。
Studies on nuclear staining by the fluorescent antibody method. I. Non-immunological factors leading to nuclear fluorescence.
作者信息
Bruchhausen D, Hofermann R, von Mayersbach H
出版信息
Immunology. 1970 Jul;19(1):1-10.
Abstract
The causes of non-specific nuclear staining in the fluorescent antibody method were investigated. Minute amounts of labelled basic proteins, present in labelled sera, stain nuclei in both direct and indirect methods. The attraction of these basic proteins is caused by electrostatical forces, binding basic items to the nuclei like basic dyes.
摘要
对荧光抗体法中非特异性核染色的原因进行了研究。标记血清中存在的微量标记碱性蛋白，在直接法和间接法中都会使细胞核染色。这些碱性蛋白的吸附是由静电力引起的，它将碱性物质像碱性染料一样与细胞核结合。
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