Ovary Z, Vaz N M, Warner N L
Immunology. 1970 Nov;19(5):715-27.
Mouse antisera to DNP conjugates were fractionated on agar block electrophoresis, and each fraction was tested for ability to give anaphylactic reactions in mice and guinea-pigs, and on mouse mast cells. Concentrations of IgG and IgG globulin were measured for each fraction, and the results indicate that mouse IgG globulin mediates both the PCA reaction in the mouse and the mast cell sensitization. PCA reactions in guinea-pigs with mouse antisera are mediated only by electrophoretic fractions containing IgG globulin. PCA reactions in guinea-pigs could be blocked by the addition of IgG myeloma containing sera to the sensitizing anti-DNP preparation, but not by addition of IgG or IgG myeloma sera. From six different IgG myeloma sera tested on their capacity to block PCA reactions in mice, five showed strong inhibitory activity whereas the sixth was inactive. However, also IgG and IgG myelomas were found capable of blocking PCA reactions in mice. The passive sensitization of mouse mast cells by purified mouse anti-DNP antibodies could also be blocked by the addition of specific anti-IgG sera. Possible interpretations of these findings are discussed.
将抗二硝基苯酚（DNP）偶联物的小鼠抗血清进行琼脂块电泳分级分离，然后检测每一级分在小鼠和豚鼠中引发过敏反应的能力以及对小鼠肥大细胞的作用。测定每一级分中IgG和IgG球蛋白的浓度，结果表明小鼠IgG球蛋白介导小鼠体内的被动皮肤过敏反应（PCA反应）以及肥大细胞致敏过程。用小鼠抗血清在豚鼠中引发的PCA反应仅由含有IgG球蛋白的电泳级分介导。向致敏的抗DNP制剂中加入含IgG骨髓瘤的血清可阻断豚鼠中的PCA反应，但加入IgG或IgG骨髓瘤血清则不能阻断。对六种不同的IgG骨髓瘤血清阻断小鼠PCA反应的能力进行检测，其中五种表现出强烈的抑制活性，而第六种则无活性。然而，也发现IgG和IgG骨髓瘤能够阻断小鼠中的PCA反应。加入特异性抗IgG血清也可阻断纯化的小鼠抗DNP抗体对小鼠肥大细胞的被动致敏。文中讨论了这些发现的可能解释。
