Lichtenstein L M, Levy D A, Ishizaka K
Immunology. 1970 Nov;19(5):831-42.
Leucocytes from allergic and most normal human donors release histamine when challenged with antibodies against human γ-globulin. This reaction (reversed anaphylaxis) is due primarily to anti-IgE antibodies although there is some response in most donors to antisera against IgG even after it has been absorbed with light and ε chains. The anti-IgE is, however, several 100-fold more potent than the anti-IgG. By passive sensitization of the leucocytes of a normal donor with serum from a ragweed-allergic patient it was shown that the normal cells became sensitive to anti-IgE and ragweed antigen E at the same time; in both cases, there was an inverse relationship between the serum concentration used for passive sensitization and the concentration of antigen or antibody required for histamine release. There is a rough correlation ( = 0.42; <0.01) between the serum IgE concentration and the response of leucocytes from allergic donors to anti-IgE and an excellent correlation ( = 0.82; <0.01) between the response of the cells to ragweed antigen E and anti-IgE. There is also a strong parallel between the mechanism of direct, antigen mediated histamine release and the reversed reaction induced by anti-IgE. Both appear to be non-serum requiring, non-cytotoxic, secretory-like responses which are inhibited by theophylline, cyclic AMP and colchicine. These data suggest that cell bound IgE is of major importance in the anaphylactic response and that the direct and reversed anaphylactic reactions both operate through cell-bound IgE and share a common reaction mechanism.
来自过敏的和大多数正常人类供体的白细胞在受到抗人γ-球蛋白抗体攻击时会释放组胺。这种反应（反向过敏反应）主要是由于抗IgE抗体引起的，尽管大多数供体对抗IgG抗血清也有一些反应，即使该抗血清已被轻链和ε链吸收。然而，抗IgE的效力比抗IgG强几百倍。通过用豚草过敏患者的血清对正常供体的白细胞进行被动致敏，结果表明正常细胞同时对抗IgE和豚草抗原E变得敏感；在这两种情况下，用于被动致敏的血清浓度与组胺释放所需的抗原或抗体浓度之间呈反比关系。过敏供体的血清IgE浓度与白细胞对抗IgE的反应之间存在粗略的相关性（r = 0.42；P<0.01），细胞对豚草抗原E和抗IgE的反应之间存在极好的相关性（r = 0.82；P<0.01）。直接的抗原介导的组胺释放机制与抗IgE诱导的反向反应机制之间也有很强的相似性。两者似乎都是非血清依赖性、非细胞毒性的分泌样反应，可被茶碱、环磷酸腺苷和秋水仙碱抑制。这些数据表明，细胞结合的IgE在过敏反应中起主要作用，直接和反向过敏反应均通过细胞结合的IgE起作用，并共享共同的反应机制。
