Revoltella R, Ovary Z
Immunology. 1969 Jul;17(1):45-54.
Mice of several strains were immunized with DNP-haemocyanin either with Al(OH) or with Freund's complete adjuvant. Antibodies of immunoglobulin type γ and γM were detected by passive haemolysis, γ antibodies by PCA in guinea-pigs, γ antibodies by PCA in mice using 2 hours of SP, and reaginic antibodies by PCA in mice using 72 hours of SP. Three of the six strains tested produced reaginic antibodies, i.e. SWR/J, C57BL/6J and A/HeJ, but only when Al(OH) was used as adjuvant. All six strains produced γ antibodies. Reaginic antibodies were separated by chromatography on DEAE-cellulose column.
用二硝基苯基血蓝蛋白分别与氢氧化铝或弗氏完全佐剂对几种品系的小鼠进行免疫。通过被动溶血检测免疫球蛋白γ型和γM型抗体，通过豚鼠体内的被动皮肤过敏反应（PCA）检测γ抗体，通过在小鼠体内使用2小时致敏期（SP）的PCA检测γ抗体，以及通过在小鼠体内使用72小时致敏期（SP）的PCA检测反应素抗体。所测试的六个品系中有三个产生了反应素抗体，即SWR/J、C57BL/6J和A/HeJ，但仅在使用氢氧化铝作为佐剂时产生。所有六个品系都产生了γ抗体。反应素抗体通过在二乙氨基乙基纤维素柱上进行色谱分离。
