Singh Anusuiya, Palma Toloza Carolyn, Muñoz María Victoria Riquelme, Carvajal Guevara Andrea
Departamento Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Santiago, Chile.
Universidad Central de Chile, Toesca, 1783, Santiago, Chile.
Bioprocess Biosyst Eng. 2025 Nov;48(11):1939-1948. doi: 10.1007/s00449-025-03207-4. Epub 2025 Sep 24.
Wheat straw is a renewable biomass with potential for bioethanol and biorefinery applications, offering potential value-added products such as enzymes and oligosaccharides. However, its complex lignocellulosic structure, costly pretreatment requirements, and formation of inhibitory compounds hinder its utilization. Moreover, commercial enzymes used in saccharification are expensive, highlighting the need for efficient in-house enzyme production. This study investigates the application of a biological pretreatment using Trametes versicolor as an eco-friendly and cost-effective method to enhance cellulose content in wheat straw. The pretreated biomass was analyzed via acid hydrolysis and employed as a substrate for cellulase production by Penicillium chrysogenum through solid-state fermentation (SSF). The liquid extract obtained after washing the biomass was evaluated for laccase and manganese peroxidase (MnP) activities. In addition, acid hydrolysis was performed to detect oligosaccharides. Biological pretreatment increased cellulose content from 36.24 ± 1.74 to 41.25 ± 1.65% and reduced lignin from 28.66 ± 1.08 to 21.12 ± 1.22%, confirming effective delignification. The pretreated straw supported cellulase production with activities of 2.66 ± 0.044 U/g (FPU), 20.77 ± 1.91 U/g (BGL), and 75.02 ± 2.48 U/g (CMC). Also, xylooligosaccharides reached 1.15 ± 0.06 g/L on day 21. These results demonstrate the potential of combining biological pretreatment and SSF as a sustainable approach to enhance enzyme yields and recover oligosaccharides for biorefinery applications.
小麦秸秆是一种可再生生物质，具有用于生物乙醇和生物精炼应用的潜力，可提供酶和低聚糖等潜在增值产品。然而，其复杂的木质纤维素结构、昂贵的预处理要求以及抑制性化合物的形成阻碍了其利用。此外，糖化过程中使用的商业酶价格昂贵，凸显了高效内部酶生产的必要性。本研究调查了使用云芝进行生物预处理作为一种环保且经济高效的方法来提高小麦秸秆中纤维素含量的应用。通过酸水解对预处理后的生物质进行分析，并将其用作产黄青霉通过固态发酵（SSF）生产纤维素酶的底物。对洗涤生物质后获得的液体提取物进行漆酶和锰过氧化物酶（MnP）活性评估。此外，进行酸水解以检测低聚糖。生物预处理使纤维素含量从36.24±1.74%提高到41.25±1.65%，木质素从28.66±1.08%降低到21.12±1.22%，证实了有效的脱木质素作用。预处理后的秸秆支持纤维素酶的生产，其活性分别为2.66±0.044 U/g（FPU）、20.77±1.91 U/g（BGL）和75.02±2.48 U/g（CMC）。此外，在第21天木寡糖达到1.15±0.06 g/L。这些结果证明了将生物预处理和固态发酵相结合作为一种可持续方法来提高酶产量并回收用于生物精炼应用的低聚糖的潜力。
