Babazadeh Beheshteh, Hatami Nemati Homeira, Arsalani Nasser, Dehghan Gholamreza, Radbin Sama, Madatova Valida
Department of Zoology and Physiology, Faculty of Biology, Baku State University, Baku, Azerbaijan.
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Vet Res Forum. 2025;16(7):399-406. doi: 10.30466/vrf.2024.2032844.4326. Epub 2025 Jul 15.
Diazinon (DZN) is a widely used organophosphate. We studied the effect of quercetin pegylated liposome (QPEGL) on acute low dose DZN-induced oxidative stress and behavioral disorders through monitoring brain serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) in mature male rats. Animals were treated in two control groups that received a single dose of normal saline and dimethyl sulfoxide, and four groups that received a single dose of DZN 10.00 mg kg (DZN), DZN 10.00 mg kg + quercetin 20.00 mg kg, DZN 10.00 mg kg + PEGL 20.00 mg kg, DZN 10.00 mg kg + QPEGL 20.00 mg kg (QPEGL), respectively. Performances of the rats were investigated by the open field and elevated plus maze tests. Twenty-four hr after the treatments, animals' brains were harvested and frozen at - 80.00 ˚C. Brain tissues 5-HIAA level was determined by the enzyme-linked immunosorbent assay. Furthermore, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels were determined for oxidative stress analysis. The motor activity was significantly reduced in the DZN group compared to the control group following increased anxiety-like behavior and ameliorated by QPEGL. Moreover, 5-HIAA and MDA levels notably increased in the DZN group compared to the control group and significantly decreased in the QPEGL group compared to the DZN group. The SOD and GPx contents were not significantly changed in the DZN group compared to the control; although, these parameters improved after treatment with QPEGL. Acute low dose DZN exposure resulted in lipid peroxidation and elevated levels of the serotonin metabolite (5-HIAA), leading to neurobehavioral disorders, such as anxiety-like behavior and impaired motor activity, which were alleviated by QPEGL.
二嗪农（DZN）是一种广泛使用的有机磷酸酯。我们通过监测成年雄性大鼠脑中血清素代谢物5-羟吲哚乙酸（5-HIAA），研究了槲皮素聚乙二醇化脂质体（QPEGL）对急性低剂量DZN诱导的氧化应激和行为障碍的影响。动物被分为两个对照组，分别接受单剂量的生理盐水和二甲基亚砜，以及四个组，分别接受单剂量的10.00 mg/kg DZN（DZN组）、10.00 mg/kg DZN + 20.00 mg/kg槲皮素、10.00 mg/kg DZN + 20.00 mg/kg聚乙二醇化脂质体（PEGL）、10.00 mg/kg DZN + 20.00 mg/kg QPEGL（QPEGL组）。通过旷场试验和高架十字迷宫试验研究大鼠的行为表现。处理后24小时，采集动物的大脑并在-80.00˚C下冷冻。通过酶联免疫吸附测定法测定脑组织中5-HIAA的水平。此外，测定丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的水平以进行氧化应激分析。与对照组相比，DZN组的运动活动显著降低，同时焦虑样行为增加，而QPEGL可改善这种情况。此外，与对照组相比，DZN组的5-HIAA和MDA水平显著升高，与DZN组相比，QPEGL组显著降低。与对照组相比，DZN组的SOD和GPx含量没有显著变化；尽管如此，用QPEGL治疗后这些参数有所改善。急性低剂量DZN暴露导致脂质过氧化和血清素代谢物（5-HIAA）水平升高，导致神经行为障碍，如焦虑样行为和运动活动受损，而QPEGL可缓解这些症状。
