胰腺脱氧核糖核酸酶的作用：将DNA激活为DNA聚合酶的模板引物的条件。
Action of pancreatic DNase: requirements for activation of DNA as a template-primer for DNA polymerase.
作者信息
Baril E, Mitchener J, Lee L, Baril B
出版信息
Nucleic Acids Res. 1977 Aug;4(8):2641-53. doi: 10.1093/nar/4.8.2641.
Abstract
Pancreatic DNase requires both Ca2+ and Mg2+ for its activity as measured by formation of an activated DNA template for in vitro DNA polymerase alpha assay and by the hyperchromic shift. Mn2+ can partially satisfy the Mg2+ requirement of the DNase for activation of DNA but the resulting template is only 50% as active in the DNA polymerase assay. When precautions are taken to avoid divalent ion contamination, pancreatic DNase is not active in the presence of Ca2+ or Mg2+ alone. analysis of the DNA by sucrose gradient centrifugation shows that only in the presence of Ca2+ plus Mg2+ or Mn2+ does pancreatic DNase produce extensive strand breaks in the DNA. The activated DNA template that yields maximal DNA polymerase activity is low molecular weight material of 30,000 to 50,000 daltons.
摘要
胰腺DNA酶的活性需要Ca2+和Mg2+，这是通过形成用于体外DNA聚合酶α测定的活化DNA模板以及增色效应来衡量的。Mn2+可以部分满足DNA酶激活DNA对Mg2+的需求，但所得模板在DNA聚合酶测定中的活性仅为50%。当采取预防措施避免二价离子污染时，胰腺DNA酶在单独存在Ca2+或Mg2+时无活性。通过蔗糖梯度离心对DNA进行分析表明，只有在存在Ca2+加Mg2+或Mn2+时，胰腺DNA酶才会在DNA中产生广泛的链断裂。产生最大DNA聚合酶活性的活化DNA模板是分子量为30,000至50,000道尔顿的低分子量物质。
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