面包酵母半胱氨酸转移核糖核酸的核苷酸序列。用胰核糖核酸酶和核糖核酸酶T1完全消化的产物。
The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1.
作者信息
Holness N J, Atfield G
出版信息
Biochem J. 1976 Feb 1;153(2):437-46. doi: 10.1042/bj1530437.
Abstract
- The nucleotide chain of tRNA Cys from baker's yeast was readily split at the anticolon into two large fragments by brief treatment with ribonuclease T1.2. The whole molecule and the two derived large fragments were completely digested with (a) pancreatic ribonuclease and (b) ribonuclease T1. The fragments present in each of the digests were separated and sequenced by conventional methods. 3. The groups of fragments derived from the two methods of digestion were entirely compatible with each other. 4. The molecule is 75 nucleotides long, but, as isolated, lacks the terminal adenosine and the neighboring cytidylic acid residue. The minor nucleotides 1-methyladenylic acid, 7-methylguanylic acid, 5-methylcytidylic acid and N6 (gamma gamma-dimethylallyl)adenylic acid (isopentenyladenylic acid) were identified.
摘要
用核糖核酸酶T1对面包酵母的半胱氨酸转运核糖核酸（tRNA Cys）的核苷酸链进行短暂处理后，其反密码子处很容易被裂解为两个大片段。
用（a）胰核糖核酸酶和（b）核糖核酸酶T1对整个分子及两个衍生的大片段进行完全消化。通过常规方法对每种消化产物中的片段进行分离和测序。
两种消化方法得到的片段组完全相互匹配。
该分子长度为75个核苷酸，但分离得到时缺少末端腺苷和相邻的胞苷酸残基。鉴定出了少量核苷酸1-甲基腺苷酸、7-甲基鸟苷酸、5-甲基胞苷酸和N6（γγ-二甲基烯丙基）腺苷酸（异戊烯基腺苷酸）。
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