Trends in Prevalence of Yeast Species Associated With Urogenital Infection in Nsukka, Nigeria: An Overview of True Species and Genotyping of -Heterozygous Isolates.

Pathogenic drug-resistant yeast species, associated with urogenital infections, are still not well-recognized in routine clinical laboratories. This study describes the prevalence and antifungal susceptibility profile of fungal species isolated from patients with urogenital infection in Nsukka, Nigeria. A total of 248 urogenital samples (voided urine, high vaginal swabs, urethral swabs, and semen) were cultured on specific mycological media for the isolation and presumptive identification of and other yeast species. Further identification of fungal isolates was performed using conventional phenotypic techniques and molecular methods. Disk diffusion and broth dilution methods were used for the antifungal susceptibility study and determination of minimum inhibitory concentration (MIC), respectively. A total of 129 were isolated from 117 patients with urogenital infection. (73.64%) was the most prevalent species followed by () (9.30%) and (6.20%), while () and exhibited the same frequency of occurrence (5.43% each). All isolates were susceptible to voriconazole and nystatin, while reduced susceptibility to fluconazole was noted. All the germ tube-positive isolates were confirmed to be by molecular methods although 15 of them were found to be heterozygous at 1 locus. This study describes the distribution of true species causing urogenital infection in Nigeria and the level of susceptibility of these species to common antifungal drugs emphasizing the need for yeast culture and antifungal susceptibility testing as part of the routine test in medical diagnostic laboratories for the proper management of urogenital candidiasis.