Yoshinaka Y, Luftig R B
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3446-50. doi: 10.1073/pnas.74.8.3446.
Disruption of Rauscher leukemia virus (RLV) with low levels of Nonidet P-40 yielded "immature" cores. These cores have a diameter of about 920 A, as opposed to the 1300-A diameter of RLV, possess knob-like protuberances, and contain a concentrically coiled internal strand apposed to the core shell. The two major polypeptide components of immature cores are (i) p30, the 30,000-dalton group-specific antigen, and (ii) a polypeptide that has the size and antigenic characteristics of P70, the 70,000-dalton precursor protein of the group-specific antigens of murine leukemia virus. Disruption of RLV at high ratios of Nonidet P-40 to virus yielded "mature" cores. These cores have an average diameter of 850 A, a smooth proteinaceous perimeter, and a collapsed internal strand, and they contain predominantly p30. Treatment of RLV with low levels of Nonidet P-40 for 16 hr at 22 degrees yielded cores that showed (I) a 70% decrease in the number of immature forms and concomitant increase in the number of mature forms, (II) a 60-90% decrease of P70, and (iii) a 30% increase in a 40,000- to 42,000-dalton protein. These results suggest that maturation of RLV cores is accomplished by cleavage of P70.
用低浓度的诺乃洗涤剂P - 40裂解劳舍尔白血病病毒（RLV）可产生“不成熟”核心。这些核心的直径约为920埃，与直径为1300埃的RLV不同，具有瘤状突起，并含有一条与核心壳相对的同心盘绕的内部链。不成熟核心的两个主要多肽成分是：（i）p30，即30000道尔顿的群特异性抗原；（ii）一种多肽，其大小和抗原特性与P70相同，P70是鼠白血病病毒群特异性抗原的70000道尔顿前体蛋白。用高比例的诺乃洗涤剂P - 40与病毒裂解RLV可产生“成熟”核心。这些核心的平均直径为850埃，有一个光滑的蛋白质表面和一条塌陷的内部链，且主要含有p30。在22摄氏度下用低浓度的诺乃洗涤剂P - 40处理RLV 16小时，产生的核心显示：（I）不成熟形式的数量减少70%，同时成熟形式的数量增加；（II）P70减少60 - 90%；（III）一种40000至42000道尔顿的蛋白质增加30%。这些结果表明RLV核心的成熟是通过P70的裂解来完成的。
