Filshtein Alina P, Belova Vlada S, Kuzmich Alexandra S, Romanenko Lyudmila A, Kokoulin Maxim S
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
Advance Engineering School "Institute of Biotechnology, Bioengineering and Food Systems", Far Eastern Federal University, 10 Ajax Bay, Russky Island, Vladivostok 690922, Russia.
Mar Drugs. 2025 Aug 28;23(9):345. doi: 10.3390/md23090345.
The lipooligosaccharide (LOS) of the marine bacterium KMM 3897 was structurally characterized using chemical analysis, NMR spectroscopy, and MALDI-TOF mass spectrometry. The oligosaccharide core consists of a monophosphorylated trisaccharide containing 2-amino-2-deoxy-D-glucose, D--D--heptose, and 3-deoxy-D--oct-2-ulosonic acid. The penta-acylated lipid A moiety features a glucosamine disaccharide backbone with phosphate groups and amide- and ester-linked primary fatty acids [i11:0 (3-OH)], along with a secondary acyl chain (i11:0 or 11:0). Immunostimulatory assays revealed that KMM 3897 LOS induced significantly weaker cytokine production in human peripheral blood mononuclear cells (PBMCs) compared with LPS. Notably, it exhibited potent antagonistic activity against LPS-mediated toxicity and suppressed caspase-4 activation in LPS-treated PBMCs. These findings highlight its anti-inflammatory and protective properties.
利用化学分析、核磁共振光谱和基质辅助激光解吸电离飞行时间质谱对海洋细菌KMM 3897的脂寡糖（LOS）进行了结构表征。寡糖核心由一个单磷酸化三糖组成，该三糖含有2-氨基-2-脱氧-D-葡萄糖、D-甘油-D-甘露庚糖和3-脱氧-D-甘露辛-2-酮糖酸。五酰化脂质A部分的特征是具有磷酸基团以及酰胺键和酯键连接的伯脂肪酸[i11:0（3-OH）]的葡糖胺二糖主链，还有一条仲酰基链（i11:0或11:0）。免疫刺激试验表明，与脂多糖（LPS）相比，KMM 3897 LOS在人外周血单核细胞（PBMC）中诱导的细胞因子产生明显较弱。值得注意的是，它对LPS介导的毒性表现出强大的拮抗活性，并抑制了LPS处理的PBMC中半胱天冬酶-4的激活。这些发现突出了其抗炎和保护特性。
