Madeira Pedro P, Uversky Vladimir N, Zaslavsky Boris Y
I3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Department of Molecular Medicine and Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Subcell Biochem. 2025;109:69-105. doi: 10.1007/978-3-032-03370-3_4.
The behavior of biomolecules in a crowded environment is affected by a number of factors, with the excluded volume being traditionally considered as a major contributor to the macromolecular crowding effects in polymer solutions. In addition to the hard nonspecific steric interactions representing the excluded volume effects, other commonly considered factors are the so-called soft interactions between the crowder and the crowdee, such as electrostatic, hydrophobic, and van der Waals interactions. However, the changes in the solvent properties of aqueous media induced by the crowding agents may represent important but commonly neglected factor affecting behavior of biomolecules in a crowded environment. This chapter systematically represents information in support to this hypothesis. Presented data clearly show that the crowding agents do induce changes in solvent properties of aqueous media in crowded environment. Therefore, these changes should be taken into account for crowding effect analysis.
生物分子在拥挤环境中的行为受到多种因素的影响，传统上，排除体积被认为是聚合物溶液中大分子拥挤效应的主要贡献因素。除了代表排除体积效应的硬的非特异性空间相互作用外，其他通常考虑的因素是拥挤剂与被拥挤物之间的所谓软相互作用，如静电、疏水和范德华相互作用。然而，拥挤剂引起的水性介质溶剂性质的变化可能是影响生物分子在拥挤环境中行为的重要但常被忽视的因素。本章系统地展示了支持这一假设的信息。所呈现的数据清楚地表明，拥挤剂确实会在拥挤环境中引起水性介质溶剂性质的变化。因此，在分析拥挤效应时应考虑这些变化。
Zotero 插件