Nandi Shishir Kumar, Hossain Arman, Nasren Shamima, Kabir Muhammad Anamul, Mamun Md Abdullah Al
Department of Aquaculture, Faculty of Fisheries, Sylhet Agricultural University, Sylhet, Bangladesh.
Laboratory of Fish Diseases Diagnosis and Pharmacology, Department of Fish Health Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet, Bangladesh.
Vet Med Sci. 2025 Nov;11(6):e70624. doi: 10.1002/vms3.70624.
The intensification of aqua-farming practices has increased the need for a constant oxygen supply to avert oxygen depletion and unexpected fish mortality in captivity. This study assessed the effects of bio-ox (10% sodium carbonate and hydrogen peroxide) as an immediate oxygen booster on water quality, behavioural patterns and cumulative per cent survival (CPS) of Puntius sophore (3.50 ± 0.13 g) in a controlled rearing environment. The completely randomized design comprised four treatments: a control group (T1) without bio-ox and three experimental groups (T2, T3 and T4) treated with 1, 3 and 5 g of bio-ox per 70 L of water, respectively. Bio-ox was applied at the start and at 36 h, with observations recorded every 6 h. Initially, water quality parameters showed no significant differences (p > 0.05) among treatments. Post-supplementation, bio-ox levels significantly influenced (p < 0.05) hydrological variables, including conductivity, total dissolved solid (TDS), total ammonia nitrogen (TAN), dissolved oxygen (DO) and pH. Conductivity and TDS increased with higher bio-ox dosages, whereas TAN levels were significantly lower (p < 0.05) in all bio-ox-treated groups compared to the control, with no significant differences among treated groups at 42, 48 and 54 h. DO peaked at 11.90 ± 0.10 mg/L at 60 h in T4, whereas T2 maintained optimal levels. The pH also varied significantly (p < 0.05), reaching a maximum of 9.23 ± 0.01 at 36 h in T4. Temperature, water pressure and salinity remained unaffected (p > 0.05). Behavioural observations indicated minimal abnormalities in T2, whereas higher bio-ox doses (T3 and T4) resulted in pronounced changes, including hyperactivity, abnormal swimming and convulsions. Kaplan-Meier survival analysis revealed significant differences among treatments, with T2 showed the highest CPS of 66.7%, which was significantly greater than that of T3 (26.7%, p = 0.026) and T4 (13.3%, p = 0.007). The control group T1 (40%) exhibited intermediate survival rates, which were not significantly different from any treatment groups. These findings suggest that bio-ox at 1 g/70 L (T2) provides optimal conditions, stabilizing fish behaviour and significantly improving survival compared to higher doses.
水产养殖活动的强化增加了持续供氧的需求，以避免圈养环境中的氧气耗尽和意外的鱼类死亡。本研究评估了生物氧（10%碳酸钠和过氧化氢）作为即时增氧剂，在可控养殖环境中对索氏无须魮（3.50±0.13克）的水质、行为模式和累积存活率（CPS）的影响。完全随机设计包括四个处理组：一个不使用生物氧的对照组（T1）和三个分别用每70升水1克、3克和5克生物氧处理的实验组（T2、T3和T4）。在开始时和36小时时施用生物氧，每6小时记录一次观察结果。最初，各处理组之间的水质参数没有显著差异（p>0.05）。添加生物氧后，生物氧水平对水文变量有显著影响（p<0.05），包括电导率、总溶解固体（TDS）、总氨氮（TAN）、溶解氧（DO）和pH值。电导率和TDS随着生物氧剂量的增加而升高，而与对照组相比，所有生物氧处理组的TAN水平显著较低（p<0.05），在42、48和54小时时处理组之间没有显著差异。在T4组中，溶解氧在60小时时达到峰值11.90±0.10毫克/升，而T2组维持在最佳水平。pH值也有显著变化（p<0.05），在T4组中，36小时时达到最大值9.23±0.01。温度、水压和盐度不受影响（p>0.05）。行为观察表明，T2组的异常情况最少，而较高的生物氧剂量（T3和T4）导致明显变化，包括多动、异常游泳和抽搐。Kaplan-Meier生存分析显示各处理组之间存在显著差异，T2组的累积存活率最高，为66.7%，显著高于T3组（26.7%，p=0.026）和T4组（13.3%，p=0.007）。对照组T1（40%）的存活率处于中间水平，与任何处理组均无显著差异。这些结果表明，每70升水1克的生物氧（T2）提供了最佳条件，与较高剂量相比，能稳定鱼类行为并显著提高存活率。
