Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, Karnataka, 575002, India.
Department of Aquaculture, College of Fisheries, Chhattisgarh Kamdhenu Vishwavidyalaya, Kawardha, Chhattisgarh, 491995, India.
Biol Trace Elem Res. 2021 Aug;199(8):3073-3088. doi: 10.1007/s12011-020-02416-0. Epub 2020 Oct 6.
In the present work, a total 180 monosex male Nile tilapia fingerlings (15.73 ± 0.05 g) were stocked in 150-l FRP tanks categorised into four diet groups with triplicate each and fed with dietary nano-selenium-supplemented diets at different concentration (T1-0, T2-0.5, T3-1.0 and T4-2.0 mg/kg of feed) for 90 days and different nutrition physiological parameters (feed utilization, haematology, serum biochemistry), immune response and antioxidant were analysed during pre- and post-challenge against Aeromonas hydrophila. The study results depicted that significantly (p < 0.05) better growth and feed utilization (absolute weight gain, specific growth rate, average daily gain, protein efficiency ratio, food conversion ratio) found in fish fed diet supplemented with 1 mg/kg of nano-Se. Significantly (p < 0.05) improved haematological (red blood cells, haemoglobin, white blood cells, platelets) and serum biochemical parameters (alanine aminotransferase, aspartate aminotransferase, glucose, cholesterol, triglycerides) observed in the same diet group. The same trend was followed by immune parameters (nitro blue tetrazolium, lysozyme activity, myeloperoxidase, total immunoglobulin). Also observed the statistically (p < 0.05) improved antioxidant activities (catalase, superoxide dismutase, glutathione peroxides, glutathione reductase, glutathione S-transferase, malondialdehyde, total antioxidant capacity) in the same diet group. Relative percent survival after the fishes challenged with A. hydrophila was significantly (p < 0.05) differed. The findings suggested that supplementation of 1 mg/kg of dietary nano-Se could able to ameliorate nutrition physiology, immunity, antioxidant activity and disease resistance in tilapia and proved that it may be one of the best element for fish farmers to increase the production in an economically feasible way.
在本工作中，将总共 180 尾单性尼罗罗非鱼鱼苗（15.73±0.05g）饲养在 150-L FRP 水槽中，分为四组饲料组，每组有三个重复，并分别用不同浓度（T1-0、T2-0.5、T3-1.0 和 T4-2.0mg/kg 饲料）的膳食纳米硒补充饲料喂养 90 天，并在对抗嗜水气单胞菌之前和之后分析不同的营养生理参数（饲料利用率、血液学、血清生化）、免疫反应和抗氧化剂。研究结果表明，在喂养添加 1mg/kg 纳米硒的饲料的鱼中，生长和饲料利用率（绝对体重增加、特定生长率、平均日增重、蛋白质效率比、食物转化率）显著提高（p<0.05）。在同一饲料组中观察到血液学（红细胞、血红蛋白、白细胞、血小板）和血清生化参数（丙氨酸氨基转移酶、天冬氨酸氨基转移酶、葡萄糖、胆固醇、甘油三酯）显著提高（p<0.05）。免疫参数（硝基蓝四唑、溶菌酶活性、髓过氧化物酶、总免疫球蛋白）也呈现相同趋势。在同一饲料组中，还观察到抗氧化活性（过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽 S-转移酶、丙二醛、总抗氧化能力）显著提高（p<0.05）。在鱼类受到嗜水气单胞菌感染后，相对存活率显著不同（p<0.05）。研究结果表明，膳食纳米硒补充 1mg/kg 可以改善罗非鱼的营养生理、免疫、抗氧化活性和抗病能力，并证明它可能是鱼类养殖户以经济可行的方式增加产量的最佳元素之一。
