Ma Shu, Liang QianXi, Li Zhili, He Feng, Wang Ye, Zhuang Zhanwei, Zhou Jian, Zhao Yunxiang, Chen Fumei
College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Foshan University, Foshan 528000, China.
College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Anim Reprod Sci. 2025 Nov;282:107998. doi: 10.1016/j.anireprosci.2025.107998. Epub 2025 Sep 22.
This study evaluated virgin coconut oil (VCO) as a natural cryoprotectant for boar sperm preservation. Duroc boar semen was cryopreserved with varying VCO concentrations (0.00 %, 0.01 %, 0.05 %, 0.10 %, 0.50 %, and 1.00 %). Comprehensive analysis revealed 0.1 % VCO optimally maintained post-thaw sperm quality, significantly improving motility, membrane integrity, acrosome integrity, and mitochondrial potential compared to controls. It also enhanced the activity of antioxidant enzymes, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD). The optimal concentration was then used in artificial insemination (AI) trials to assess in vivo fertility. Metabolomic analysis identified significant alterations in lipid and energy metabolism pathways, with particular changes in N-acetylneuraminic acid and lysophosphatidylcholines that correlated with sperm quality improvements. These findings demonstrate VCO's effectiveness in protecting boar sperm during cryopreservation through multiple mechanisms including membrane stabilization, oxidative protection, and metabolic regulation. The 0.1 % VCO supplementation presents a practical solution for swine artificial insemination programs, offering both cryoprotective benefits and potential cost advantages over conventional additives.
本研究评估了初榨椰子油（VCO）作为公猪精液冷冻保存的天然冷冻保护剂的效果。用不同浓度的VCO（0.00%、0.01%、0.05%、0.10%、0.50%和1.00%）对杜洛克公猪精液进行冷冻保存。综合分析表明，0.1%的VCO能最佳地维持解冻后精子质量，与对照组相比，显著提高了精子活力、膜完整性、顶体完整性和线粒体电位。它还增强了抗氧化酶谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）的活性。然后将最佳浓度用于人工授精（AI）试验以评估体内受精能力。代谢组学分析确定了脂质和能量代谢途径的显著变化，特别是N-乙酰神经氨酸和溶血磷脂酰胆碱的变化与精子质量改善相关。这些发现表明，VCO通过膜稳定、氧化保护和代谢调节等多种机制在冷冻保存过程中对公猪精子具有保护作用。添加0.1%的VCO为猪人工授精计划提供了一个切实可行的解决方案，与传统添加剂相比，它既具有冷冻保护作用，又具有潜在的成本优势。
