Tran Khuong Thi Thanh, Lu San Nguyen Cam, Nguyen Duy Lam Khanh
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Can Tho City, 900000, Vietnam.
Department of Biology - Biotechnology, University of Science, Vietnam National University Ho Chi Minh City, Ho Chi Minh City, 700000, Vietnam.
Vet Res Commun. 2025 Sep 27;49(6):332. doi: 10.1007/s11259-025-10878-7.
Oxidative stress induced by environmental toxicants such as Bisphenol A (BPA) and cryopreservation significantly impairs sperm quality in goats, leading to reduced viability, motility, membrane integrity, and increased DNA fragmentation. This study aimed to evaluate the protective effects of curcumin, a natural polyphenolic antioxidant on goat sperm exposed to BPA and cryopreservation stress in vitro. Three experiments were conducted using semen collected from healthy adult goats. In Experiment 1, sperm samples were treated with 30 µM BPA to induce oxidative damage, followed by curcumin supplementation at 0, 5, 10, and 20 µM. In Experiment 2, freshly collected semen was cryopreserved in liquid nitrogen (- 196 °C) with the same curcumin concentrations added to the freezing extender. In Experiment 3, commercial frozen goat semen straws were thawed in media supplemented with curcumin at identical concentrations. Each treatment was replicated 12 times. BPA exposure significantly decreased overall motility and progressive motility compared to control. Treatment with 10 µM curcumin effectively restored sperm quality. In cryopreserved samples, 5 µM curcumin provided optimal protection. Similarly, in thawed commercial semen, 5 µM curcumin significantly improved sperm performance. These results demonstrate that curcumin at optimal concentrations effectively mitigates oxidative stress-induced damage to goat sperm, whether from BPA toxicity or cryopreservation. The dual role of curcumin during both freezing and thawing underscores its practical value as an antioxidant additive in semen preservation protocols. Its inclusion could enhance sperm quality, promote fertility outcomes, and support reproductive biotechnology applications in animal breeding programs.
双酚A（BPA）等环境毒物以及冷冻保存所诱导的氧化应激会显著损害山羊的精子质量，导致活力、运动能力、膜完整性降低以及DNA碎片化增加。本研究旨在评估天然多酚类抗氧化剂姜黄素对体外暴露于BPA和冷冻保存应激的山羊精子的保护作用。使用从健康成年山羊采集的精液进行了三项实验。在实验1中，精子样本用30µM BPA处理以诱导氧化损伤，随后分别添加0、5、10和20µM的姜黄素。在实验2中，将新鲜采集的精液在液氮（-196°C）中冷冻保存，在冷冻稀释液中添加相同浓度的姜黄素。在实验3中，将市售冷冻山羊精液细管在添加相同浓度姜黄素的培养基中解冻。每个处理重复12次。与对照组相比，BPA暴露显著降低了总体运动能力和渐进性运动能力。用10µM姜黄素处理可有效恢复精子质量。在冷冻保存的样本中，5µM姜黄素提供了最佳保护。同样，在解冻的市售精液中，5µM姜黄素显著改善了精子性能。这些结果表明，最佳浓度的姜黄素可有效减轻氧化应激对山羊精子造成的损伤，无论是来自BPA毒性还是冷冻保存。姜黄素在冷冻和解冻过程中的双重作用凸显了其作为精液保存方案中抗氧化添加剂的实际价值。加入姜黄素可以提高精子质量，促进生育结果，并支持动物育种计划中的生殖生物技术应用。
