Zhang Qin, Liang Yuguan, Li Jiqing, Li Luoqing, Meng Liuqing, Zeng Qinghui, Wang Dapeng, Wang Rui, Tong Tong, Liu Yongqiang, Yang Huizan
Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, School of Marine Sciences and Biotechnology, Guangxi Minzu University, 158 University Road, Nanning 530008, China.
Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, 8 Qingshan Road, Nanning 530021, China.
Biology (Basel). 2025 Sep 1;14(9):1164. doi: 10.3390/biology14091164.
This study investigated the effects of dietary supplementation with different concentrations (0.0 g/kg, 0.1 g/kg, 1.0 g/kg, 10.0 g/kg) on red claw crayfish (). Four groups were established: control group (CK, 0.0 g/kg), low-dose group (HL, 0.1 g/kg), medium-dose group (HM, 1.0 g/kg), and high-dose group (HH, 10.0 g/kg). The feeding trial lasted for 56 days. The results showed that, compared with the control group, all supplementation groups exhibited significantly reduced feed conversion ratios ( < 0.05). The HM and HH groups demonstrated significant increases in body length growth rate, specific growth rate, weight gain rate, hepatosomatic index, and survival rate ( < 0.05). All supplemented groups showed significantly enhanced trypsin and lipase activities in intestines and trypsin activity in the hepatopancreas ( < 0.05). The HM and HH groups exhibited elevated α-amylase activity in the hepatopancreas ( < 0.05). Compared with the control group, marine red yeast supplementation reduced colonization of potential pathogens while increasing probiotic abundance, effectively improving intestinal microbiota structure. The HM group significantly improved intestinal villus length, width, and muscular thickness ( < 0.05). All supplemented groups showed considerable upregulation of hepatopancreatic genes related to immunity (heat shock protein 70, down syndrome cell adhesion molecule, crustacean antibacterial peptide, serine proteinase inhibitors, crustacean hyperglycemic hormone, anti-lipopolysaccharide factor, lysozyme, and alkaline phosphatase) and antioxidant defense (superoxide dismutase, glutathione peroxidase, glutathione, and catalase) ( < 0.05). These findings indicate that can significantly enhance digestive enzyme activity, maintain intestinal health, improve antioxidant and immune-related gene expression, and promote growth performance in red claw crayfish, with the HM group (1.0 g/kg ) showing optimal promotion effects.
本研究调查了不同浓度（0.0克/千克、0.1克/千克、1.0克/千克、10.0克/千克）的膳食补充剂对红螯螯虾的影响。设立了四组：对照组（CK，0.0克/千克）、低剂量组（HL，0.1克/千克）、中剂量组（HM，1.0克/千克）和高剂量组（HH，10.0克/千克）。投喂试验持续了56天。结果表明，与对照组相比，所有补充剂组的饲料转化率均显著降低（P<0.05）。HM组和HH组的体长增长率、特定生长率、增重率、肝体指数和存活率显著提高（P<0.05）。所有补充剂组肠道中的胰蛋白酶和脂肪酶活性以及肝胰腺中的胰蛋白酶活性均显著增强（P<0.05）。HM组和HH组肝胰腺中的α-淀粉酶活性升高（P<0.05）。与对照组相比，添加海洋红酵母减少了潜在病原体的定殖，同时增加了益生菌丰度，有效改善了肠道微生物群结构。HM组显著改善了肠道绒毛长度、宽度和肌肉厚度（P<0.05）。所有补充剂组肝胰腺中与免疫相关的基因（热休克蛋白70、唐氏综合征细胞粘附分子、甲壳类抗菌肽、丝氨酸蛋白酶抑制剂、甲壳类高血糖激素、抗脂多糖因子、溶菌酶和碱性磷酸酶）和抗氧化防御相关的基因（超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽和过氧化氢酶）均显著上调（P<0.05）。这些发现表明，[具体物质未明确]可以显著提高消化酶活性，维持肠道健康，改善抗氧化和免疫相关基因的表达，并促进红螯螯虾的生长性能，其中HM组（1.0克/千克[具体物质未明确]）显示出最佳的促进效果。
