Chen Dongsheng, Pei Honglin, Chen Yuchen, Liu Anzheng, Xing Tengyu, Zhang Hai, Wang Luo
Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China.
Biology (Basel). 2025 Sep 11;14(9):1246. doi: 10.3390/biology14091246.
Collagen content is a primary indicator of quality traits in aquatic animals, with dietary supplementation currently being the main approach to enhance collagen levels. However, the pathways by which food-derived components mediate host collagen synthesis via the gut microbiota remain unclear. This study investigated the regulatory role of gut microbiota in collagen synthesis within the body wall of the sea cucumber () under dietary supplementation. The results showed that the groups supplemented with 0.60% choline (DJ), 0.50% betaine (TC), and 2.75% glycine (G) significantly increased the collagen content in the sea cucumber body wall by 8.82%, 21.28%, and 22.13%, respectively, compared to the control group (NC). The composition and metabolic function of the sea cucumber gut microbiota were altered by dietary supplementation. The dominant gut microbiota in the supplemented group were , , , and , which possess capabilities in amino acid metabolism and the decomposition of organic carbon and nitrogen sources. In addition, metabolic pathways such as amino acid metabolism, carbohydrate metabolism, energy metabolism, and nucleotide metabolism were significantly enriched. Glycine and other key collagen precursors exhibited significantly elevated levels in the gut of supplemented sea cucumbers. Research indicates that dietary supplementation with choline, betaine, and glycine modulates the composition and function of the gut microbiota in sea cucumbers. This supplementation also promotes the accumulation of collagen precursors and influences collagen content in the body wall. The objective of this study is to provide a theoretical basis to enhance the quality and efficiency of the sea cucumber aquaculture industry.
胶原蛋白含量是水生动物品质性状的主要指标，目前通过饮食补充是提高胶原蛋白水平的主要方法。然而，食物来源成分通过肠道微生物群介导宿主胶原蛋白合成的途径仍不清楚。本研究调查了饮食补充条件下肠道微生物群对海参（）体壁内胶原蛋白合成的调节作用。结果表明，与对照组（NC）相比，添加0.60%胆碱（DJ）、0.50%甜菜碱（TC）和2.75%甘氨酸（G）的组海参体壁中的胶原蛋白含量分别显著增加了8.82%、21.28%和22.13%。饮食补充改变了海参肠道微生物群的组成和代谢功能。补充组中占主导地位的肠道微生物是、、和，它们具有氨基酸代谢以及有机碳和氮源分解的能力。此外，氨基酸代谢、碳水化合物代谢、能量代谢和核苷酸代谢等代谢途径显著富集。甘氨酸和其他关键胶原蛋白前体在补充海参的肠道中表现出显著升高的水平。研究表明，饮食中补充胆碱、甜菜碱和甘氨酸可调节海参肠道微生物群的组成和功能。这种补充还促进胶原蛋白前体的积累，并影响体壁中的胶原蛋白含量。本研究的目的是为提高海参养殖业的质量和效率提供理论依据。
