Wang Lijun, Li Maolong, Liu Chaoqi, Li Xinxin, Wang Ping, Chang Juan, Jin Sanjun, Yin Qingqiang, Zhu Qun, Dang Xiaowei, Lu Fushan
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Henan Delin Biological Product Co., Ltd., Xinxiang 453000, China.
Animals (Basel). 2025 Sep 19;15(18):2746. doi: 10.3390/ani15182746.
The potential of fungal probiotics as ruminant feed additives has not been extensively studied. This study aimed to evaluate the effect of and supplementation on Angus cattle during the early stages of fattening. In this study, 80 Angus cattle aged approximately 9~10 months (40 males and 40 females), with an average initial body weight (BW) of 276.46 ± 27.92 kg, were randomly assigned to four groups. Each group included 4 replicates (2 replicates of males and 2 replicates of females). Each replicate contained 5 male or 5 female Angus cattle. Cattle in the control group received a total mixed ration (TMR) without additives, while those in test groups 1, 2, and 3 received a TMR supplemented with complex probiotics (CPs) at 1.0, 2.0, and 3.0 g·kg of feed dry matter (DM), respectively. The adaptation and experimental periods were 7 and 60 days, respectively. Compared with those in the control group, the apparent digestibility of ether extract, calcium, and acid detergent fiber (ADF) was higher in test group 3. Test group 2 exhibited increased apparent digestibility of hemicellulose. Meanwhile, test group 3 exhibited increased levels of acetate, propionate, butyrate, and total volatile fatty acids and decreased pH. CPs increased the abundance of the phylum, , , and and decreased the proportions of , , and . was associated with the apparent digestibility of CP, Ca, and ADF. was associated with increased apparent digestibility of CP, ADF, and hemicellulose. This suggests that CPs improve crude protein and cellulose digestion by increasing the proportions of and . Therefore, the optimal CP dietary supplementation dose for Angus cattle was 3 g·kg of DM.
真菌益生菌作为反刍动物饲料添加剂的潜力尚未得到广泛研究。本研究旨在评估在育肥初期添加[具体物质1]和[具体物质2]对安格斯牛的影响。本研究中，80头年龄约9至10个月的安格斯牛（40头雄性和40头雌性），平均初始体重（BW）为276.46±27.92千克，被随机分为四组。每组包括4个重复（2个雄性重复和2个雌性重复）。每个重复包含5头雄性或5头雌性安格斯牛。对照组的牛只接受不含添加剂的全混合日粮（TMR），而试验组1、2和3的牛只分别接受添加了1.0、2.0和3.0克·千克饲料干物质（DM）复合益生菌（CPs）的TMR。适应期和试验期分别为7天和60天。与对照组相比，试验组3中乙醚提取物、钙和酸性洗涤纤维（ADF）的表观消化率更高。试验组2中半纤维素的表观消化率有所提高。同时，试验组3中乙酸、丙酸、丁酸和总挥发性脂肪酸水平升高，pH值降低。CPs增加了[门1]、[门2]、[门3]和[门4]的丰度，并降低了[门5]、[门6]和[门7]的比例。[菌属1]与CP、钙和ADF的表观消化率相关。[菌属2]与CP、ADF和半纤维素表观消化率的提高相关。这表明CPs通过增加[菌属1]和[菌属2]的比例来改善粗蛋白和纤维素的消化。因此，安格斯牛的最佳CP日粮添加剂量为3克·千克DM。
