Molecular and Serological Detection of spp. in Mediterranean Wild Carnivores and Feral Cats: Implications for Wildlife Health and One Health Surveillance.

Leishmaniasis is a zoonotic disease caused by protozoan parasites of the genus , transmitted by phlebotomine sandflies. While domestic dogs are the main hosts in the Mediterranean basin, wild carnivores have also been proposed as potential reservoirs. This study assessed the presence of spp. in 250 animals from the Valencian Community, eastern Spain-an endemic region-using TaqMan qPCR on spleen samples from 216 wild carnivores and sera from 34 feral cats, and ELISA serology on 174 wild carnivores. DNA of spp. was detected in 14 out of 250 individuals (5.6%), with red foxes representing most positive cases (10/102; 9.8%). Seropositivity was observed only in red foxes, with 5 out of 174 individuals testing positive (2.9%). Most qPCR-positive animals had high Ct values, consistent with low parasite loads. One fox, positive by both methods, showed advanced skin lesions and was later diagnosed with sarcoptic mange, suggesting possible interaction with infection. The overall low prevalence and parasite burden suggest limited circulation in the surveyed wildlife. These findings contribute to understanding the epidemiological role of wild mesocarnivores and highlight the relevance of wildlife monitoring within a One Health approach.