Etaka Cyril A, Silva Eugenia M, Hamilton Alexis M, Murphy Claire M, Strawn Laura K
Department of Food Science and Technology, Virginia Tech, Blacksburg, VA 24061, USA.
School of Food Science, Washington State University Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, USA.
Foods. 2025 Sep 18;14(18):3247. doi: 10.3390/foods14183247.
Short-season (90 d) produce packing operations may run double shifts with no clean breaks in between. This practice can result in produce contamination from food contact surfaces that are not cleaned and sanitized. Our study examined the survival of and on polycarbonate, polypropylene, polyvinyl chloride (PVC), rubber, and stainless steel surfaces that contact produce in operations that have a short packing season. Coupons were spot-inoculated with five-strain cocktails of rifampicin-resistant or (~7 log CFU/coupon), stored at 22 °C and 45-55% relative humidity, and enumerated at 0, 0.06, 0.25, 1, 2, 3, 7, 10, 14, 21, 30, 60, and 90 d. Significant differences were evaluated ( ≤ 0.05), and survival was modeled using linear and biphasic models. reductions varied significantly by surface type, with rubber showing the greatest survival, followed by stainless steel at 90 d. In contrast, concentrations on polycarbonate, polypropylene, and PVC were below the limit of detection at 90 d. reductions were not significantly different across materials at 90 d. Biphasic models better fit the inactivation of both pathogens. These findings highlight the importance of clean breaks and focusing interventions where pathogens demonstrate greater persistence in short-season packinghouses.
短季(90天)农产品包装作业可能会两班倒,期间没有彻底的清洁间隔。这种做法可能导致农产品受到未清洁和消毒的食品接触表面的污染。我们的研究考察了在包装季较短的作业中,与农产品接触的聚碳酸酯、聚丙烯、聚氯乙烯(PVC)、橡胶和不锈钢表面上[具体两种病原体未给出,暂用“和”代替]的存活情况。将试片用耐利福平的或[具体两种病原体未给出,暂用“和”代替]的五菌株混合菌液(约7 log CFU/试片)进行点接种,在22℃和45 - 55%相对湿度下储存,并在0、0.06、0.25、1、2、3、7、10、14、21、30、60和90天进行计数。评估显著差异(P≤0.05),并使用线性和双相模型对存活情况进行建模。[具体两种病原体未给出,暂用“和”代替]的减少量因表面类型而异,橡胶表面的存活量最大,其次是90天时的不锈钢表面。相比之下,聚碳酸酯、聚丙烯和PVC表面上[具体两种病原体未给出,暂用“和”代替]的浓度在90天时低于检测限。90天时,[具体两种病原体未给出,暂用“和”代替]在不同材料上的减少量没有显著差异。双相模型更能拟合两种病原体的失活情况。这些发现凸显了彻底清洁间隔的重要性,以及在短季包装厂中病原体持续存在时间较长的地方集中采取干预措施的重要性。