Zheng Huowang, Shi Fangshu, Li Jinjun, Bian Xiangyu, Wu Shuisheng, Li Xiaoqiong
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Foods. 2025 Sep 19;14(18):3255. doi: 10.3390/foods14183255.
This study systematically investigated the dose-response relationship of resistant starch type 2 (RS2; Hi-maize 260; 0-15 g/L) on gut microbial composition, short-chain fatty acid (SCFA)/gas output, and tryptophan catabolism using an in vitro fermentation model. The highest RS2 concentration (15 g/L) elicited optimal metabolic outcomes, including maximal SCFA production; significant HS reduction; and redirected tryptophan metabolism from potentially detrimental indoles toward neuroprotective metabolites. Microbial profiling revealed dose-dependent enrichment of saccharolytic taxa (, ) with concomitant suppression of proteolytic pathobionts (e.g., ). Correlation analyses revealed strong positive associations between beneficial microbes and both SCFAs and neuroprotective metabolites, whereas pathogenic taxa correlated inversely with these compounds. Collectively, these findings establish that functionally relevant microbiome modulation requires a sufficiently high, dose-tailored intake of RS2, providing a rational basis for precision dietary strategies aimed at improving host metabolic and gut health.
本研究使用体外发酵模型，系统地研究了2型抗性淀粉（RS2；高直链玉米淀粉260；0 - 15 g/L）对肠道微生物组成、短链脂肪酸（SCFA）/气体产生量以及色氨酸分解代谢的剂量反应关系。最高RS2浓度（15 g/L）产生了最佳代谢结果，包括最大程度的SCFA产生；显著降低硫化氢；并将色氨酸代谢从潜在有害的吲哚转向具有神经保护作用的代谢产物。微生物分析显示，糖分解类群（ ， ）呈剂量依赖性富集，同时蛋白水解致病共生菌（如 ）受到抑制。相关性分析显示，有益微生物与SCFAs和神经保护代谢产物之间存在强正相关，而致病类群与这些化合物呈负相关。总体而言，这些发现表明，功能性相关的微生物群调节需要足够高的、根据剂量定制的RS2摄入量，为旨在改善宿主代谢和肠道健康的精准饮食策略提供了合理依据。
